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Deletion-mutant epidermal growth factor receptor in human gliomas: effects of type II mutation on receptor function.

Publication ,  Journal Article
Humphrey, PA; Gangarosa, LM; Wong, AJ; Archer, GE; Lund-Johansen, M; Bjerkvig, R; Laerum, OD; Friedman, HS; Bigner, DD
Published in: Biochem Biophys Res Commun
August 15, 1991

Malignant human glioma D-298 MG amplifies a rearranged epidermal growth factor receptor (EGFR) gene (c-erbB proto-oncogene), resulting in an in-frame deletion of 83 amino acids in domain IV of the extracellular domain of the EGFR. EGF and transforming growth factor-a (TGF-a) bound to the mutant EGFR with high affinity and enhanced the intrinsic mutant EGFR kinase activity. The mutant EGFR was capable of transducing EGF-stimulated glioma cell proliferation and invasiveness in an in vitro three-dimensional spheroid model. The deletion-mutant EGFR in D-298 MG is capable of being activated by growth factor; this suggests that overexpression of this mutant EGFR protein rather than structural alteration may be the more significant biologic event.

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Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 15, 1991

Volume

178

Issue

3

Start / End Page

1413 / 1420

Location

United States

Related Subject Headings

  • Transforming Growth Factor alpha
  • Receptor, erbB-2
  • Receptor, ErbB-2
  • Proto-Oncogenes
  • Proto-Oncogene Proteins
  • Proto-Oncogene Mas
  • Protein-Tyrosine Kinases
  • Phosphorylation
  • Molecular Sequence Data
  • Kinetics
 

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Humphrey, P. A., Gangarosa, L. M., Wong, A. J., Archer, G. E., Lund-Johansen, M., Bjerkvig, R., … Bigner, D. D. (1991). Deletion-mutant epidermal growth factor receptor in human gliomas: effects of type II mutation on receptor function. Biochem Biophys Res Commun, 178(3), 1413–1420. https://doi.org/10.1016/0006-291x(91)91051-d
Humphrey, P. A., L. M. Gangarosa, A. J. Wong, G. E. Archer, M. Lund-Johansen, R. Bjerkvig, O. D. Laerum, H. S. Friedman, and D. D. Bigner. “Deletion-mutant epidermal growth factor receptor in human gliomas: effects of type II mutation on receptor function.Biochem Biophys Res Commun 178, no. 3 (August 15, 1991): 1413–20. https://doi.org/10.1016/0006-291x(91)91051-d.
Humphrey PA, Gangarosa LM, Wong AJ, Archer GE, Lund-Johansen M, Bjerkvig R, et al. Deletion-mutant epidermal growth factor receptor in human gliomas: effects of type II mutation on receptor function. Biochem Biophys Res Commun. 1991 Aug 15;178(3):1413–20.
Humphrey, P. A., et al. “Deletion-mutant epidermal growth factor receptor in human gliomas: effects of type II mutation on receptor function.Biochem Biophys Res Commun, vol. 178, no. 3, Aug. 1991, pp. 1413–20. Pubmed, doi:10.1016/0006-291x(91)91051-d.
Humphrey PA, Gangarosa LM, Wong AJ, Archer GE, Lund-Johansen M, Bjerkvig R, Laerum OD, Friedman HS, Bigner DD. Deletion-mutant epidermal growth factor receptor in human gliomas: effects of type II mutation on receptor function. Biochem Biophys Res Commun. 1991 Aug 15;178(3):1413–1420.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 15, 1991

Volume

178

Issue

3

Start / End Page

1413 / 1420

Location

United States

Related Subject Headings

  • Transforming Growth Factor alpha
  • Receptor, erbB-2
  • Receptor, ErbB-2
  • Proto-Oncogenes
  • Proto-Oncogene Proteins
  • Proto-Oncogene Mas
  • Protein-Tyrosine Kinases
  • Phosphorylation
  • Molecular Sequence Data
  • Kinetics