Deletion-mutant epidermal growth factor receptor in human gliomas: effects of type II mutation on receptor function.

Journal Article (Journal Article)

Malignant human glioma D-298 MG amplifies a rearranged epidermal growth factor receptor (EGFR) gene (c-erbB proto-oncogene), resulting in an in-frame deletion of 83 amino acids in domain IV of the extracellular domain of the EGFR. EGF and transforming growth factor-a (TGF-a) bound to the mutant EGFR with high affinity and enhanced the intrinsic mutant EGFR kinase activity. The mutant EGFR was capable of transducing EGF-stimulated glioma cell proliferation and invasiveness in an in vitro three-dimensional spheroid model. The deletion-mutant EGFR in D-298 MG is capable of being activated by growth factor; this suggests that overexpression of this mutant EGFR protein rather than structural alteration may be the more significant biologic event.

Full Text

Duke Authors

Cited Authors

  • Humphrey, PA; Gangarosa, LM; Wong, AJ; Archer, GE; Lund-Johansen, M; Bjerkvig, R; Laerum, OD; Friedman, HS; Bigner, DD

Published Date

  • August 15, 1991

Published In

Volume / Issue

  • 178 / 3

Start / End Page

  • 1413 - 1420

PubMed ID

  • 1678600

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/0006-291x(91)91051-d


  • eng

Conference Location

  • United States