Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas.

Published

Journal Article

We have previously demonstrated antibody production to a glioma-associated variant form of the human epidermal growth factor receptor in rabbits that had received a synthetic peptide mimicking the unique primary structure of the variant protein as immunogen. We report here the response of mice, rabbits, goats, and macaques immunized by various protocols to this peptide. Titers to both peptide- and cell-elaborated variant receptor were measured, and the capacity to recognize the variant receptor in human tumor samples was determined. Within the range of species and strains investigated, we demonstrated a variable species-associated response to the peptide (rabbits > mice > goats > rats > macaques). Rabbits and a single goat produced specific, high titer antibody activity to the variant receptor protein following immunization with peptide alone. Murine titers to the parent protein were not appreciable following peptide immunization alone; additional immunization with variant receptor as expressed on cell membranes was used to boost this response.

Full Text

Duke Authors

Cited Authors

  • Wikstrand, CJ; Stanley, SD; Humphrey, PA; Pegram, CN; Archer, GE; Kurpad, S; Shibuya, M; Bigner, DD

Published Date

  • July 1993

Published In

Volume / Issue

  • 46 / 1-2

Start / End Page

  • 165 - 173

PubMed ID

  • 8360327

Pubmed Central ID

  • 8360327

International Standard Serial Number (ISSN)

  • 0165-5728

Digital Object Identifier (DOI)

  • 10.1016/0165-5728(93)90246-u

Language

  • eng

Conference Location

  • Netherlands