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Radioiodination of a monoclonal antibody using N-succinimidyl 5-iodo-3-pyridinecarboxylate.

Publication ,  Journal Article
Garg, S; Garg, PK; Zhao, XG; Friedman, HS; Bigner, DD; Zalutsky, MR
Published in: Nucl Med Biol
October 1993

The potential utility of N-succinimidyl 5-iodo-3-pyridinecarboxylate (SIPC) for the radioiodination of monoclonal antibodies was investigated. Paired-label studies were performed using the anti-tenascin antibody 81C6 in athymic mice bearing subcutaneous D-54 MG human glioma xenografts. Radiolabeling was also done using N-succinimidyl 3-iodobenzoate (SIB). Radioiodination of SIPC and SIB both proceeded in 60-80% yield, but protein coupling efficiencies with SIB were higher (76 +/- 16 vs 60 +/- 7%). Immunoreactivity and affinity of both preparations were similar. Using SIPC, thyroid uptake was quite low, decreasing from 0.3% at day 1 to 0.05% at day 8. Tumor uptake reached 46 +/- 11% injected dose/g at day 1 but declined gradually thereafter. This apparent decline reflected the rapid growth of these xenografts since tumor accumulation expressed as percentage of injected dose remained nearly constant up to day 9. These results suggest that SIPC, like SIB, offers significant advantages for labeling antibodies when compared with conventional protein iodination methods.

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Published In

Nucl Med Biol

DOI

ISSN

0969-8051

Publication Date

October 1993

Volume

20

Issue

7

Start / End Page

835 / 842

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Tissue Distribution
  • Succinimides
  • Nuclear Medicine & Medical Imaging
  • Nicotinic Acids
  • Neoplasm Transplantation
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Isotope Labeling
 

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Garg, S., Garg, P. K., Zhao, X. G., Friedman, H. S., Bigner, D. D., & Zalutsky, M. R. (1993). Radioiodination of a monoclonal antibody using N-succinimidyl 5-iodo-3-pyridinecarboxylate. Nucl Med Biol, 20(7), 835–842. https://doi.org/10.1016/0969-8051(93)90149-o
Garg, S., P. K. Garg, X. G. Zhao, H. S. Friedman, D. D. Bigner, and M. R. Zalutsky. “Radioiodination of a monoclonal antibody using N-succinimidyl 5-iodo-3-pyridinecarboxylate.Nucl Med Biol 20, no. 7 (October 1993): 835–42. https://doi.org/10.1016/0969-8051(93)90149-o.
Garg S, Garg PK, Zhao XG, Friedman HS, Bigner DD, Zalutsky MR. Radioiodination of a monoclonal antibody using N-succinimidyl 5-iodo-3-pyridinecarboxylate. Nucl Med Biol. 1993 Oct;20(7):835–42.
Garg, S., et al. “Radioiodination of a monoclonal antibody using N-succinimidyl 5-iodo-3-pyridinecarboxylate.Nucl Med Biol, vol. 20, no. 7, Oct. 1993, pp. 835–42. Pubmed, doi:10.1016/0969-8051(93)90149-o.
Garg S, Garg PK, Zhao XG, Friedman HS, Bigner DD, Zalutsky MR. Radioiodination of a monoclonal antibody using N-succinimidyl 5-iodo-3-pyridinecarboxylate. Nucl Med Biol. 1993 Oct;20(7):835–842.
Journal cover image

Published In

Nucl Med Biol

DOI

ISSN

0969-8051

Publication Date

October 1993

Volume

20

Issue

7

Start / End Page

835 / 842

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Tissue Distribution
  • Succinimides
  • Nuclear Medicine & Medical Imaging
  • Nicotinic Acids
  • Neoplasm Transplantation
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Isotope Labeling