In vivo growth conditions suppress the expression of ganglioside GM2 and favour that of lacto series gangliosides in the human glioma D-54MG cell line.

Published

Journal Article

The human glioma D-54MG cell line grown in vitro primarily expresses ganglio series gangliosides, particularly GM2. Subcutaneous injection of these cells into nude mice produced xenografts with an increased content of the human glioma-associated lacto series gangliosides, primarily 3'-isoLM1, an alteration that was dose dependent, with the highest dose (1 x 10(8)) resulting in a phenotype that was most like that of the inoculum. After one passage in vivo, the lacto series dominated and reached a proportional level that was kept throughout the 10 passages. The mRNA levels of the GM2-synthase clearly coincided with GM2 expression and was 20 times higher in cells grown in vitro than in those grown in vivo. These results support the view that ganglioside expression in human gliomas is strongly influenced by environmental factors.

Full Text

Duke Authors

Cited Authors

  • Fredman, P; Wikstrand, CJ; Månsson, JE; Reifenberger, G; Bigner, SH; Rasheed, A; Svennerholm, L; Bigner, DD

Published Date

  • June 1996

Published In

Volume / Issue

  • 13 / 3

Start / End Page

  • 391 - 399

PubMed ID

  • 8781970

Pubmed Central ID

  • 8781970

International Standard Serial Number (ISSN)

  • 0282-0080

Language

  • eng

Conference Location

  • United States