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In vivo growth conditions suppress the expression of ganglioside GM2 and favour that of lacto series gangliosides in the human glioma D-54MG cell line.

Publication ,  Journal Article
Fredman, P; Wikstrand, CJ; Månsson, JE; Reifenberger, G; Bigner, SH; Rasheed, A; Svennerholm, L; Bigner, DD
Published in: Glycoconj J
June 1996

The human glioma D-54MG cell line grown in vitro primarily expresses ganglio series gangliosides, particularly GM2. Subcutaneous injection of these cells into nude mice produced xenografts with an increased content of the human glioma-associated lacto series gangliosides, primarily 3'-isoLM1, an alteration that was dose dependent, with the highest dose (1 x 10(8)) resulting in a phenotype that was most like that of the inoculum. After one passage in vivo, the lacto series dominated and reached a proportional level that was kept throughout the 10 passages. The mRNA levels of the GM2-synthase clearly coincided with GM2 expression and was 20 times higher in cells grown in vitro than in those grown in vivo. These results support the view that ganglioside expression in human gliomas is strongly influenced by environmental factors.

Duke Scholars

Published In

Glycoconj J

DOI

ISSN

0282-0080

Publication Date

June 1996

Volume

13

Issue

3

Start / End Page

391 / 399

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Transcription, Genetic
  • Spectrometry, Mass, Fast Atom Bombardment
  • RNA, Messenger
  • Polypeptide N-acetylgalactosaminyltransferase
  • N-Acetylgalactosaminyltransferases
  • Molecular Sequence Data
  • Mice, Nude
  • Mice
 

Citation

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Fredman, P., Wikstrand, C. J., Månsson, J. E., Reifenberger, G., Bigner, S. H., Rasheed, A., … Bigner, D. D. (1996). In vivo growth conditions suppress the expression of ganglioside GM2 and favour that of lacto series gangliosides in the human glioma D-54MG cell line. Glycoconj J, 13(3), 391–399. https://doi.org/10.1007/BF00731472
Fredman, P., C. J. Wikstrand, J. E. Månsson, G. Reifenberger, S. H. Bigner, A. Rasheed, L. Svennerholm, and D. D. Bigner. “In vivo growth conditions suppress the expression of ganglioside GM2 and favour that of lacto series gangliosides in the human glioma D-54MG cell line.Glycoconj J 13, no. 3 (June 1996): 391–99. https://doi.org/10.1007/BF00731472.
Fredman P, Wikstrand CJ, Månsson JE, Reifenberger G, Bigner SH, Rasheed A, et al. In vivo growth conditions suppress the expression of ganglioside GM2 and favour that of lacto series gangliosides in the human glioma D-54MG cell line. Glycoconj J. 1996 Jun;13(3):391–9.
Fredman, P., et al. “In vivo growth conditions suppress the expression of ganglioside GM2 and favour that of lacto series gangliosides in the human glioma D-54MG cell line.Glycoconj J, vol. 13, no. 3, June 1996, pp. 391–99. Pubmed, doi:10.1007/BF00731472.
Fredman P, Wikstrand CJ, Månsson JE, Reifenberger G, Bigner SH, Rasheed A, Svennerholm L, Bigner DD. In vivo growth conditions suppress the expression of ganglioside GM2 and favour that of lacto series gangliosides in the human glioma D-54MG cell line. Glycoconj J. 1996 Jun;13(3):391–399.
Journal cover image

Published In

Glycoconj J

DOI

ISSN

0282-0080

Publication Date

June 1996

Volume

13

Issue

3

Start / End Page

391 / 399

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Transcription, Genetic
  • Spectrometry, Mass, Fast Atom Bombardment
  • RNA, Messenger
  • Polypeptide N-acetylgalactosaminyltransferase
  • N-Acetylgalactosaminyltransferases
  • Molecular Sequence Data
  • Mice, Nude
  • Mice