Gangliosides in the human glioma cell line U-118 MG grown in culture or as xenografts in nude rats.
This study was undertaken to characterize gangliosides in the human glioma cell line U-118 MG. The cell line was grown both in cell culture and as xenografts in nude rats. A common finding in both culture and xenograft cells was the high proportion of the lactoseries ganglioside 3'-LM1, approximately one third of the total ganglioside sialic acid. Otherwise, there were marked differences between the two cell sources. The cells grown in culture had a more simple ganglioside pattern than those grown in xenografts. In the latter instance, more complex gangliosides of the lactoseries, including 3'8'-LD1, sialyllactonorhexaosylceramide and a branched structure with two terminal NeuAc alpha 2-3Gal beta 1- 4GlcNAc chains, and the gangliotetraose series were found. Another marked difference involved GM2, which in the cultured cells was a major fraction, indicating that the synthesis of the gangliotetraose series gangliosides in the former stopped at the level of GM2. These results show that the ganglioside composition of a glioma cell line is strongly influenced by environmental factors.
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Related Subject Headings
- Tumor Cells, Cultured
- Sialic Acids
- Rats
- Neoplasm Transplantation
- Humans
- Glioma
- Gangliosides
- Chromatography, Thin Layer
- Brain
- Animals
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Tumor Cells, Cultured
- Sialic Acids
- Rats
- Neoplasm Transplantation
- Humans
- Glioma
- Gangliosides
- Chromatography, Thin Layer
- Brain
- Animals