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Gangliosides in the human glioma cell line U-118 MG grown in culture or as xenografts in nude rats.

Publication ,  Journal Article
Fredman, P; Månsson, JE; Bigner, SH; Wikstrand, CJ; Bigner, DD; Svennerholm, L
Published in: Biochim Biophys Acta
August 6, 1990

This study was undertaken to characterize gangliosides in the human glioma cell line U-118 MG. The cell line was grown both in cell culture and as xenografts in nude rats. A common finding in both culture and xenograft cells was the high proportion of the lactoseries ganglioside 3'-LM1, approximately one third of the total ganglioside sialic acid. Otherwise, there were marked differences between the two cell sources. The cells grown in culture had a more simple ganglioside pattern than those grown in xenografts. In the latter instance, more complex gangliosides of the lactoseries, including 3'8'-LD1, sialyllactonorhexaosylceramide and a branched structure with two terminal NeuAc alpha 2-3Gal beta 1- 4GlcNAc chains, and the gangliotetraose series were found. Another marked difference involved GM2, which in the cultured cells was a major fraction, indicating that the synthesis of the gangliotetraose series gangliosides in the former stopped at the level of GM2. These results show that the ganglioside composition of a glioma cell line is strongly influenced by environmental factors.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

August 6, 1990

Volume

1045

Issue

3

Start / End Page

239 / 244

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Sialic Acids
  • Rats
  • Neoplasm Transplantation
  • Humans
  • Glioma
  • Gangliosides
  • Chromatography, Thin Layer
  • Brain
  • Animals
 

Citation

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MLA
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Fredman, P., Månsson, J. E., Bigner, S. H., Wikstrand, C. J., Bigner, D. D., & Svennerholm, L. (1990). Gangliosides in the human glioma cell line U-118 MG grown in culture or as xenografts in nude rats. Biochim Biophys Acta, 1045(3), 239–244. https://doi.org/10.1016/0005-2760(90)90126-i
Fredman, P., J. E. Månsson, S. H. Bigner, C. J. Wikstrand, D. D. Bigner, and L. Svennerholm. “Gangliosides in the human glioma cell line U-118 MG grown in culture or as xenografts in nude rats.Biochim Biophys Acta 1045, no. 3 (August 6, 1990): 239–44. https://doi.org/10.1016/0005-2760(90)90126-i.
Fredman P, Månsson JE, Bigner SH, Wikstrand CJ, Bigner DD, Svennerholm L. Gangliosides in the human glioma cell line U-118 MG grown in culture or as xenografts in nude rats. Biochim Biophys Acta. 1990 Aug 6;1045(3):239–44.
Fredman, P., et al. “Gangliosides in the human glioma cell line U-118 MG grown in culture or as xenografts in nude rats.Biochim Biophys Acta, vol. 1045, no. 3, Aug. 1990, pp. 239–44. Pubmed, doi:10.1016/0005-2760(90)90126-i.
Fredman P, Månsson JE, Bigner SH, Wikstrand CJ, Bigner DD, Svennerholm L. Gangliosides in the human glioma cell line U-118 MG grown in culture or as xenografts in nude rats. Biochim Biophys Acta. 1990 Aug 6;1045(3):239–244.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

August 6, 1990

Volume

1045

Issue

3

Start / End Page

239 / 244

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Sialic Acids
  • Rats
  • Neoplasm Transplantation
  • Humans
  • Glioma
  • Gangliosides
  • Chromatography, Thin Layer
  • Brain
  • Animals