Gangliosides in the human glioma cell line U-118 MG grown in culture or as xenografts in nude rats.

Published

Journal Article

This study was undertaken to characterize gangliosides in the human glioma cell line U-118 MG. The cell line was grown both in cell culture and as xenografts in nude rats. A common finding in both culture and xenograft cells was the high proportion of the lactoseries ganglioside 3'-LM1, approximately one third of the total ganglioside sialic acid. Otherwise, there were marked differences between the two cell sources. The cells grown in culture had a more simple ganglioside pattern than those grown in xenografts. In the latter instance, more complex gangliosides of the lactoseries, including 3'8'-LD1, sialyllactonorhexaosylceramide and a branched structure with two terminal NeuAc alpha 2-3Gal beta 1- 4GlcNAc chains, and the gangliotetraose series were found. Another marked difference involved GM2, which in the cultured cells was a major fraction, indicating that the synthesis of the gangliotetraose series gangliosides in the former stopped at the level of GM2. These results show that the ganglioside composition of a glioma cell line is strongly influenced by environmental factors.

Full Text

Duke Authors

Cited Authors

  • Fredman, P; Månsson, JE; Bigner, SH; Wikstrand, CJ; Bigner, DD; Svennerholm, L

Published Date

  • August 6, 1990

Published In

Volume / Issue

  • 1045 / 3

Start / End Page

  • 239 - 244

PubMed ID

  • 2386796

Pubmed Central ID

  • 2386796

International Standard Serial Number (ISSN)

  • 0006-3002

Digital Object Identifier (DOI)

  • 10.1016/0005-2760(90)90126-i

Language

  • eng

Conference Location

  • Netherlands