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Immunoaffinity purification of human O6-alkylguanine-DNA alkyltransferase using newly developed monoclonal antibodies.

Publication ,  Journal Article
Brent, TP; von Wronski, M; Pegram, CN; Bigner, DD
Published in: Cancer Res
January 1, 1990

As well as repairing mutagenic lesions induced by simple methylating agents, O6-alkylguanine-DNA alkyltransferase repairs precursors of cytotoxic interstrand cross-links induced by chloroethylating anticancer drugs. Moreover, levels of the transferase correlate with cellular resistance to these agents. Thus far, the human transferase has not been highly purified. In our quest to obtain the homogeneous protein we have produced four stable cloned hybridomas that secrete monoclonal antibodies against the alkyltransferase from human lymphoblasts. The specificity of these monoclonals was established by immunoblot analysis and immunoprecipitation. All these antibodies recognized the alkyltransferase only after its denaturation by sodium dodecyl sulfate. One of them, designated 19.2, was used in immunoaffinity chromatography to obtain the pure protein.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

January 1, 1990

Volume

50

Issue

1

Start / End Page

58 / 61

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • O(6)-Methylguanine-DNA Methyltransferase
  • Molecular Weight
  • Mice, Inbred BALB C
  • Mice
  • Methyltransferases
  • Humans
  • Fluorescent Antibody Technique
  • Female
 

Citation

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MLA
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Brent, T. P., von Wronski, M., Pegram, C. N., & Bigner, D. D. (1990). Immunoaffinity purification of human O6-alkylguanine-DNA alkyltransferase using newly developed monoclonal antibodies. Cancer Res, 50(1), 58–61.
Brent, T. P., M. von Wronski, C. N. Pegram, and D. D. Bigner. “Immunoaffinity purification of human O6-alkylguanine-DNA alkyltransferase using newly developed monoclonal antibodies.Cancer Res 50, no. 1 (January 1, 1990): 58–61.
Brent TP, von Wronski M, Pegram CN, Bigner DD. Immunoaffinity purification of human O6-alkylguanine-DNA alkyltransferase using newly developed monoclonal antibodies. Cancer Res. 1990 Jan 1;50(1):58–61.
Brent, T. P., et al. “Immunoaffinity purification of human O6-alkylguanine-DNA alkyltransferase using newly developed monoclonal antibodies.Cancer Res, vol. 50, no. 1, Jan. 1990, pp. 58–61.
Brent TP, von Wronski M, Pegram CN, Bigner DD. Immunoaffinity purification of human O6-alkylguanine-DNA alkyltransferase using newly developed monoclonal antibodies. Cancer Res. 1990 Jan 1;50(1):58–61.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

January 1, 1990

Volume

50

Issue

1

Start / End Page

58 / 61

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • O(6)-Methylguanine-DNA Methyltransferase
  • Molecular Weight
  • Mice, Inbred BALB C
  • Mice
  • Methyltransferases
  • Humans
  • Fluorescent Antibody Technique
  • Female