Immunoaffinity purification of human O6-alkylguanine-DNA alkyltransferase using newly developed monoclonal antibodies.


Journal Article

As well as repairing mutagenic lesions induced by simple methylating agents, O6-alkylguanine-DNA alkyltransferase repairs precursors of cytotoxic interstrand cross-links induced by chloroethylating anticancer drugs. Moreover, levels of the transferase correlate with cellular resistance to these agents. Thus far, the human transferase has not been highly purified. In our quest to obtain the homogeneous protein we have produced four stable cloned hybridomas that secrete monoclonal antibodies against the alkyltransferase from human lymphoblasts. The specificity of these monoclonals was established by immunoblot analysis and immunoprecipitation. All these antibodies recognized the alkyltransferase only after its denaturation by sodium dodecyl sulfate. One of them, designated 19.2, was used in immunoaffinity chromatography to obtain the pure protein.

Full Text

Duke Authors

Cited Authors

  • Brent, TP; von Wronski, M; Pegram, CN; Bigner, DD

Published Date

  • January 1, 1990

Published In

Volume / Issue

  • 50 / 1

Start / End Page

  • 58 - 61

PubMed ID

  • 2403418

Pubmed Central ID

  • 2403418

International Standard Serial Number (ISSN)

  • 0008-5472


  • eng

Conference Location

  • United States