Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species.

Published

Journal Article

The highly reproducible histochemical localization of glial fibrillary acidic protein (GFAP)+ qualifies it as an important marker of astrocytes in both research and clinical applications. The primary objective of this study was to produce monoclonal antibodies having the advantage of invariant specificity, affinity, and titer to GFAP-specific epitopes of wide species distribution. We report here the characterization of four monoclonal antibodies that recognize the same or spatially close epitopes specific to GFAP. The epitope(s) detected has been phylogenetically conserved; human, bovine, ovine, canine, porcine, rabbit, guinea pig, rat, murine, and chicken brain homogenates all specifically absorb monoclonal antibody activity. Of importance to the routine application of these new anti-GFAP monoclonal antibodies is the demonstration here of the stability of the antigen-antibody interaction in normal, reactive, and neoplastic astrocytes of both rat and human origin following various methods of fixation.

Full Text

Duke Authors

Cited Authors

  • Pegram, CN; Eng, LF; Wikstrand, CJ; McComb, RD; Lee, YL; Bigner, DD

Published Date

  • 1985

Published In

Volume / Issue

  • 3 / 2

Start / End Page

  • 119 - 138

PubMed ID

  • 2413405

Pubmed Central ID

  • 2413405

International Standard Serial Number (ISSN)

  • 0734-600X

Language

  • eng

Conference Location

  • United States