A serially transplantable human giant cell glioblastoma that maintains a near-haploid stem line.

Journal Article (Journal Article)

We have karyotyped a human giant cell glioblastoma removed from an 11-year-old girl and have established from it a subcutaneously transplantable line in athymic nude mice. The original tumor contained near-haploid cells with 25 or 26 chromosomes, including two copies of #1, (7 or 7p+) and #18. There were also hyperdiploid (49-52) cells that were tetraploid for these same three chromosome types; doubled versions of the hyperdiploid population were also seen. The stemline of the mouse-grown tumor was 26,X, +1, +7p+, +18 in the first passage and has remained consistently near-haploid through ten serial in vivo passages. Growth stabilization has occurred with an average latency of less than 3 months. This transplantable line is available for evaluating chemotherapeutic responsiveness of human giant cell glioblastoma and for studying near-haploidy in solid human tumors.

Full Text

Duke Authors

Cited Authors

  • Bigner, SH; Mark, J; Schold, SC; Eng, LF; Bigner, DD

Published Date

  • October 1985

Published In

Volume / Issue

  • 18 / 2

Start / End Page

  • 141 - 153

PubMed ID

  • 3840409

International Standard Serial Number (ISSN)

  • 0165-4608

Digital Object Identifier (DOI)

  • 10.1016/0165-4608(85)90064-0


  • eng

Conference Location

  • United States