A serially transplantable human giant cell glioblastoma that maintains a near-haploid stem line.
Journal Article (Journal Article)
We have karyotyped a human giant cell glioblastoma removed from an 11-year-old girl and have established from it a subcutaneously transplantable line in athymic nude mice. The original tumor contained near-haploid cells with 25 or 26 chromosomes, including two copies of #1, (7 or 7p+) and #18. There were also hyperdiploid (49-52) cells that were tetraploid for these same three chromosome types; doubled versions of the hyperdiploid population were also seen. The stemline of the mouse-grown tumor was 26,X, +1, +7p+, +18 in the first passage and has remained consistently near-haploid through ten serial in vivo passages. Growth stabilization has occurred with an average latency of less than 3 months. This transplantable line is available for evaluating chemotherapeutic responsiveness of human giant cell glioblastoma and for studying near-haploidy in solid human tumors.
Full Text
Duke Authors
Cited Authors
- Bigner, SH; Mark, J; Schold, SC; Eng, LF; Bigner, DD
Published Date
- October 1985
Published In
Volume / Issue
- 18 / 2
Start / End Page
- 141 - 153
PubMed ID
- 3840409
International Standard Serial Number (ISSN)
- 0165-4608
Digital Object Identifier (DOI)
- 10.1016/0165-4608(85)90064-0
Language
- eng
Conference Location
- United States