Amplification of the c-myc gene in human medulloblastoma cell lines and xenografts.
Journal Article (Journal Article)
Cultured cell lines and xenografts derived from 7 human medulloblastomas were evaluated for amplification of the c-myc, N-myc, epidermal growth factor receptor, and gli genes by Southern blot analysis. Karyotypes of the original biopsies and early passaged cells demonstrated double minute chromosomes in 4 of the 7 cases. All 7 samples (3 cell lines and 4 xenografts) from the 4 tumors with double minute chromosomes contained amplification of the c-myc gene. Cell lines and xenografts derived from the 3 biopsies without double minute chromosomes failed to demonstrate amplification of the 4 genes which were tested, but a rearrangement of the c-myc gene occurred in 1 of the 3 tumors. These observations demonstrate that the c-myc gene is often amplified and/or rearranged in human medulloblastomas and suggest that amplification of this gene provides a growth advantage for medulloblastoma cells in vitro and in vivo.
Full Text
Duke Authors
Cited Authors
- Bigner, SH; Friedman, HS; Vogelstein, B; Oakes, WJ; Bigner, DD
Published Date
- April 15, 1990
Published In
Volume / Issue
- 50 / 8
Start / End Page
- 2347 - 2350
PubMed ID
- 2180567
International Standard Serial Number (ISSN)
- 0008-5472
Language
- eng
Conference Location
- United States