Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft.
Published
Journal Article
A methylator-resistant human glioblastoma multiforme xenograft, D-245 MG (PR), in athymic nude mice was established by serially treating the parent xenograft D-245 MG with procarbazine. D-245 MG xenografts were sensitive to procarbazine, temozolomide, N-methyl-N-nitrosourea, 1,3-bis(2-chloroethyl)-1-nitrosourea, 9-aminocamptothecin, topotecan, CPT-11, cyclophosphamide, and busulfan. D-245 MG (PR) xenografts were resistant to procarbazine, temozolomide, N-methyl-N-nitrosourea, and busulfan, but they were sensitive to the other agents. Both D-245 MG and D-245 MG (PR) xenografts displayed no O6-alkylguanine-DNA alkyltransferase activity, and their levels of glutathione and glutathione-S-transferase were similar. D-245 MG xenografts expressed the human mismatch repair proteins hMSH2 and hMLH1, whereas D-245 MG (PR) expressed hMLH1 but not hMSH2.
Full Text
Duke Authors
Cited Authors
- Friedman, HS; Johnson, SP; Dong, Q; Schold, SC; Rasheed, BK; Bigner, SH; Ali-Osman, F; Dolan, E; Colvin, OM; Houghton, P; Germain, G; Drummond, JT; Keir, S; Marcelli, S; Bigner, DD; Modrich, P
Published Date
- July 15, 1997
Published In
Volume / Issue
- 57 / 14
Start / End Page
- 2933 - 2936
PubMed ID
- 9230204
Pubmed Central ID
- 9230204
International Standard Serial Number (ISSN)
- 0008-5472
Language
- eng
Conference Location
- United States