Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft.

Journal Article

A methylator-resistant human glioblastoma multiforme xenograft, D-245 MG (PR), in athymic nude mice was established by serially treating the parent xenograft D-245 MG with procarbazine. D-245 MG xenografts were sensitive to procarbazine, temozolomide, N-methyl-N-nitrosourea, 1,3-bis(2-chloroethyl)-1-nitrosourea, 9-aminocamptothecin, topotecan, CPT-11, cyclophosphamide, and busulfan. D-245 MG (PR) xenografts were resistant to procarbazine, temozolomide, N-methyl-N-nitrosourea, and busulfan, but they were sensitive to the other agents. Both D-245 MG and D-245 MG (PR) xenografts displayed no O6-alkylguanine-DNA alkyltransferase activity, and their levels of glutathione and glutathione-S-transferase were similar. D-245 MG xenografts expressed the human mismatch repair proteins hMSH2 and hMLH1, whereas D-245 MG (PR) expressed hMLH1 but not hMSH2.

Full Text

Duke Authors

Cited Authors

  • Friedman, HS; Johnson, SP; Dong, Q; Schold, SC; Rasheed, BK; Bigner, SH; Ali-Osman, F; Dolan, E; Colvin, OM; Houghton, P; Germain, G; Drummond, JT; Keir, S; Marcelli, S; Bigner, DD; Modrich, P

Published Date

  • July 15, 1997

Published In

Volume / Issue

  • 57 / 14

Start / End Page

  • 2933 - 2936

PubMed ID

  • 9230204

International Standard Serial Number (ISSN)

  • 0008-5472

Language

  • eng

Conference Location

  • United States