Amplification and expression of the epidermal growth factor receptor gene in human glioma xenografts.

Published

Journal Article

Xenografts from eight malignant human gliomas were established in athymic mice and were used to study amplification and expression of the epidermal growth factor receptor (EGFR) gene. Tissue identity between biopsy and xenografts was confirmed by karyotypic profiles, which showed that each glioma xenograft retained structural abnormalities, including double minute chromosomes, present in the parent glioma. EGFR gene amplification was found in six of the eight glioma biopsies and their corresponding xenografts. Expression of the EGFR gene was measured by Scatchard analysis, affinity reactions, immunoprecipitations, Western immunoblots, and immunocytochemistry; significant expression of the EGFR gene was only detectable in xenografts with EGFR gene amplification. Moreover, five of the six xenografts with EGFR gene amplification demonstrated structural alterations of the EGFR gene, which was associated with low-molecular-weight EGFR proteins. These xenografts represent an excellent tissue source and in vivo model system for characterizing the epidermal growth factor receptor in malignant human gliomas.

Full Text

Duke Authors

Cited Authors

  • Humphrey, PA; Wong, AJ; Vogelstein, B; Friedman, HS; Werner, MH; Bigner, DD; Bigner, SH

Published Date

  • April 15, 1988

Published In

Volume / Issue

  • 48 / 8

Start / End Page

  • 2231 - 2238

PubMed ID

  • 3258189

Pubmed Central ID

  • 3258189

International Standard Serial Number (ISSN)

  • 0008-5472

Language

  • eng

Conference Location

  • United States