Skip to main content

Differentiation characteristics of newly established medulloblastoma cell lines (D384 Med, D425 Med, and D458 Med) and their transplantable xenografts.

Publication ,  Journal Article
He, XM; Wikstrand, CJ; Friedman, HS; Bigner, SH; Pleasure, S; Trojanowski, JQ; Bigner, DD
Published in: Lab Invest
June 1991

Three new human medulloblastoma (MB) cell lines (D384 Med, D425 Med, and D458 Med) and their transplantable xenografts were examined for antigenic expression with antibodies against neuroectodermal antigens, cytoskeletal proteins, neuroendocrine markers, glioma-associated antigens, tenascin, human lymphocyte antigen molecules, epidermal growth factor receptor, and T-cell antigen by indirect immunofluorescence, avidin-biotin complex peroxidase immunohistochemistry, and immunoblot methods. We found that each of the three cell lines expressed vimentin; low-, middle-, and high-molecular-weight neurofilament proteins; and the synaptic vesicle membrane glycoprotein synaptophysin. Each of the cell lines also reacted with antibodies against neural cell adhesion molecules, but none of them were positive for antibodies against glial fibrillary acidic protein, keratin, microtubule-associated protein tau and microtubule-associated protein 2, human lymphocyte antigen-DR, epidermal growth factor receptor, and T-cell antigen. Immunoreactivities with anti-tenascin and anti-glioma-associated antibodies were variable in these cell lines. Anti-human lymphocyte antigen-A,B and anti-beta 2-microglobulin antibodies reacted with xenografts of D384 Med and D425 Med and were weakly positive for a small population of D384 Med cultured cells. In summary, the detection of neurofilament proteins and synaptophysin and the absence of glial fibrillary acidic protein provide strong evidence for a neuronal phenotype of D384 Med, D425 Med, and D458 Med.

Duke Scholars

Published In

Lab Invest

ISSN

0023-6837

Publication Date

June 1991

Volume

64

Issue

6

Start / End Page

833 / 843

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Synaptophysin
  • Phenotype
  • Pathology
  • Neurofilament Proteins
  • Nerve Tissue Proteins
  • Neoplasm Transplantation
  • Microtubule-Associated Proteins
  • Membrane Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
He, X. M., Wikstrand, C. J., Friedman, H. S., Bigner, S. H., Pleasure, S., Trojanowski, J. Q., & Bigner, D. D. (1991). Differentiation characteristics of newly established medulloblastoma cell lines (D384 Med, D425 Med, and D458 Med) and their transplantable xenografts. Lab Invest, 64(6), 833–843.
He, X. M., C. J. Wikstrand, H. S. Friedman, S. H. Bigner, S. Pleasure, J. Q. Trojanowski, and D. D. Bigner. “Differentiation characteristics of newly established medulloblastoma cell lines (D384 Med, D425 Med, and D458 Med) and their transplantable xenografts.Lab Invest 64, no. 6 (June 1991): 833–43.
He XM, Wikstrand CJ, Friedman HS, Bigner SH, Pleasure S, Trojanowski JQ, et al. Differentiation characteristics of newly established medulloblastoma cell lines (D384 Med, D425 Med, and D458 Med) and their transplantable xenografts. Lab Invest. 1991 Jun;64(6):833–43.
He XM, Wikstrand CJ, Friedman HS, Bigner SH, Pleasure S, Trojanowski JQ, Bigner DD. Differentiation characteristics of newly established medulloblastoma cell lines (D384 Med, D425 Med, and D458 Med) and their transplantable xenografts. Lab Invest. 1991 Jun;64(6):833–843.

Published In

Lab Invest

ISSN

0023-6837

Publication Date

June 1991

Volume

64

Issue

6

Start / End Page

833 / 843

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Synaptophysin
  • Phenotype
  • Pathology
  • Neurofilament Proteins
  • Nerve Tissue Proteins
  • Neoplasm Transplantation
  • Microtubule-Associated Proteins
  • Membrane Proteins