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GD3 expression by cultured human tumor cells of neuroectodermal origin.

Publication ,  Journal Article
He, X; Wikstrand, CJ; Fredman, P; Månsson, JE; Svennerholm, L; Bigner, DD
Published in: Acta Neuropathol
1989

Seven monoclonal antibodies (mAbs) reactive with ganglioside II3(NeuAc)2-LacCer (GD3) were generated; four of these mAbs (DMAb-21, DMAb-22, DMAb-23, and DMAb-24) by immunizing mice with GD3 adsorbed to Salmonella minnesota and the remaining three (DMAb-7, DMAb-8, and DMAb-17) with melanoma line SK-MEL 28, which contains 1.4 nmol sialic acid of GD3 per mg protein. The specificities of the mAbs were defined by high-performance thin-layer chromatography (HPTLC) immunostain and solid-phase radioimmunoassay (SP-RIA) with a panel of purified gangliosides. DMAb-7 and DMAb-8 reacted with GD3, IV3(NeuAc)2nLcOse4Cer(3',8'-LD1), and very weakly with IV3(NeuAc)2II3NeuAcGgOse4Cer (GT1a), but not with II3NeuAc-LacCer (GM3), II3NeuAcGgOse3Cer(GM2), II3NeuAcGgOse4Cer (GM1), II3NeuAc, IV3NeuAcGgOse4Cer (GD1a), II3(NeuAc)2GgOse3(GD2), II3(NeuAc)2GgOse4Cer (GD1b), IV3NeuAcII3(NeuAc)2, GgOse4Cer(GT1b), suggesting the binding epitope to be a terminal tetrasaccharide NeuAc alpha 2-8NeuAc alpha 2-3Gal beta 1-4(Glc or GlcNAc). DMAb-7 and DMAb-8 were used to investigate the expression of GD3 on cultured human tumor cells of neuroectodermal origin. Thirteen of 19 gliomas, 3 of 5 medulloblastomas, 5 of 5 neuroblastomas, 2 of 2 melanomas, and 1 of 3 teratomas were shown to react with DMAb-8 and/or DMAb-7 by cell surface-RIA (CS-RIA) and immunofluorescence (IF) assays. HPTLC and densitometric analysis confirmed these results, as positive immunostains in the GD3 region were obtained with oligoganglioside fractions from 9 glioma, 1 medulloblastoma, 2 neuroblastoma, 1 melanoma, and 1 teratoma cell line. Glioma cell line U-105 MG and medulloblastoma cell line Daoy contain GD3 as shown by HPTLC immunostain analysis of extracts, although GD3 was undetectable on the cell surface as determined by CS-RIA and IF.(ABSTRACT TRUNCATED AT 250 WORDS)

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Published In

Acta Neuropathol

DOI

ISSN

0001-6322

Publication Date

1989

Volume

79

Issue

3

Start / End Page

317 / 325

Location

Germany

Related Subject Headings

  • Tumor Cells, Cultured
  • Radioimmunoassay
  • Neurology & Neurosurgery
  • Neuroblastoma
  • Mice, Inbred BALB C
  • Mice
  • Melanoma
  • Medulloblastoma
  • Male
  • Glioma
 

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He, X., Wikstrand, C. J., Fredman, P., Månsson, J. E., Svennerholm, L., & Bigner, D. D. (1989). GD3 expression by cultured human tumor cells of neuroectodermal origin. Acta Neuropathol, 79(3), 317–325. https://doi.org/10.1007/BF00294668
He, X., C. J. Wikstrand, P. Fredman, J. E. Månsson, L. Svennerholm, and D. D. Bigner. “GD3 expression by cultured human tumor cells of neuroectodermal origin.Acta Neuropathol 79, no. 3 (1989): 317–25. https://doi.org/10.1007/BF00294668.
He X, Wikstrand CJ, Fredman P, Månsson JE, Svennerholm L, Bigner DD. GD3 expression by cultured human tumor cells of neuroectodermal origin. Acta Neuropathol. 1989;79(3):317–25.
He, X., et al. “GD3 expression by cultured human tumor cells of neuroectodermal origin.Acta Neuropathol, vol. 79, no. 3, 1989, pp. 317–25. Pubmed, doi:10.1007/BF00294668.
He X, Wikstrand CJ, Fredman P, Månsson JE, Svennerholm L, Bigner DD. GD3 expression by cultured human tumor cells of neuroectodermal origin. Acta Neuropathol. 1989;79(3):317–325.
Journal cover image

Published In

Acta Neuropathol

DOI

ISSN

0001-6322

Publication Date

1989

Volume

79

Issue

3

Start / End Page

317 / 325

Location

Germany

Related Subject Headings

  • Tumor Cells, Cultured
  • Radioimmunoassay
  • Neurology & Neurosurgery
  • Neuroblastoma
  • Mice, Inbred BALB C
  • Mice
  • Melanoma
  • Medulloblastoma
  • Male
  • Glioma