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Glycolipids and glycosyltransferases in permanent cell lines established from human medulloblastomas.

Publication ,  Journal Article
Gottfries, J; Percy, AK; Månsson, JE; Fredman, P; Wikstrand, CJ; Friedman, HS; Bigner, DD; Svennerholm, L
Published in: Biochim Biophys Acta
February 5, 1991

Medulloblastoma biopsies are heterogenous and might contain normal brain tissue, which limits the usefulness of such tumor material for biochemical analyses. We have, therefore, examined the gangliosides and their metabolism using the medulloblastoma cell lines. Daoy and D341 Med, cultured both in vitro and as xenografts in nude mice. The ganglioside patterns in the Daoy showed a switch from a high GM2, 70% (mol% of total ganglioside sialic acid) and low lactoseries gangliosides (2%) content in monolayer cultures, to a high proportion of lactoseries gangliosides (50%) and virtually no GM2 (1%) in xenografts, but an increased proportion of other a-series gangliosides. The D341 Med showed a similar change regarding the lacto-series gangliosides from 1% in suspension culture to 10% in xenografts. The activity of five glycosyltransferases, GM3, GD3, GM2, GM1 and LA2 synthases, did not parallel the ganglioside patterns and could not account for the noted variations therein. In the Daoy cell line the LA2 synthase as well as the GM2 synthase activity was relatively high in both culture systems, despite the marked difference in the expression of GM2 and the lactoseries gangliosides. These results suggest that environmental factors play a crucial role for the in vivo activity of the glycosyltransferases.

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Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

February 5, 1991

Volume

1081

Issue

3

Start / End Page

253 / 261

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Sialyltransferases
  • Polypeptide N-acetylgalactosaminyltransferase
  • N-Acetylglucosaminyltransferases
  • N-Acetylgalactosaminyltransferases
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Humans
  • Glycolipids
 

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Gottfries, J., Percy, A. K., Månsson, J. E., Fredman, P., Wikstrand, C. J., Friedman, H. S., … Svennerholm, L. (1991). Glycolipids and glycosyltransferases in permanent cell lines established from human medulloblastomas. Biochim Biophys Acta, 1081(3), 253–261. https://doi.org/10.1016/0005-2760(91)90279-q
Gottfries, J., A. K. Percy, J. E. Månsson, P. Fredman, C. J. Wikstrand, H. S. Friedman, D. D. Bigner, and L. Svennerholm. “Glycolipids and glycosyltransferases in permanent cell lines established from human medulloblastomas.Biochim Biophys Acta 1081, no. 3 (February 5, 1991): 253–61. https://doi.org/10.1016/0005-2760(91)90279-q.
Gottfries J, Percy AK, Månsson JE, Fredman P, Wikstrand CJ, Friedman HS, et al. Glycolipids and glycosyltransferases in permanent cell lines established from human medulloblastomas. Biochim Biophys Acta. 1991 Feb 5;1081(3):253–61.
Gottfries, J., et al. “Glycolipids and glycosyltransferases in permanent cell lines established from human medulloblastomas.Biochim Biophys Acta, vol. 1081, no. 3, Feb. 1991, pp. 253–61. Pubmed, doi:10.1016/0005-2760(91)90279-q.
Gottfries J, Percy AK, Månsson JE, Fredman P, Wikstrand CJ, Friedman HS, Bigner DD, Svennerholm L. Glycolipids and glycosyltransferases in permanent cell lines established from human medulloblastomas. Biochim Biophys Acta. 1991 Feb 5;1081(3):253–261.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

February 5, 1991

Volume

1081

Issue

3

Start / End Page

253 / 261

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Sialyltransferases
  • Polypeptide N-acetylgalactosaminyltransferase
  • N-Acetylglucosaminyltransferases
  • N-Acetylgalactosaminyltransferases
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Humans
  • Glycolipids