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Immunobiology of primary intracranial tumors. Part 8: Serological responses to active immunization of patients with anaplastic gliomas.

Publication ,  Journal Article
Mahaley, MS; Gillespie, GY; Gillespie, RP; Watkins, PJ; Bigner, DD; Wikstrand, CJ; MacQueen, JM; Sanfilippo, F
Published in: J Neurosurg
August 1983

Serial serological studies were carried out on 19 of 20 patients with malignant gliomas who were actively immunized with one of two human glioma tissue culture cell lines (D-54MG or U-251MG). Most patients mounted a significant serum reaction to histocompatibility antigens (HLA's), as well as an antibody response to fetal bovine serum (FBS) which was added to the glioma-cell inoculum. These two sources of antibody accounted for greater than 90% of the antibody induced by these inoculations. Two patients continued to have significant amounts of binding antibody to the original immunizing cell line following exhaustive absorptions of FBS and these two had all remaining significant antibody removed by further absorption of the serum against the 2-T osteogenic sarcoma tissue culture cell line known to possess antigens cross-reactive with human gliomas. One single patient continued to show significant antibody binding to the original glioma cell line following absorption against FBS, human platelets, and the 2-T cell line, and therefore seems to have produced glioma-distinctive antibodies in response to immunization. The antibody preparation from this patient was also cytotoxic against the original glioma cell line, as well as another recently cultured human glioblastoma cell line. The significance of these serological studies is discussed as it relates to immunological responses patients with gliomas may make to active immunization.

Duke Scholars

Published In

J Neurosurg

DOI

ISSN

0022-3085

Publication Date

August 1983

Volume

59

Issue

2

Start / End Page

208 / 216

Location

United States

Related Subject Headings

  • Neurology & Neurosurgery
  • Lymphocytes
  • Immunization
  • Humans
  • HLA Antigens
  • Glioma
  • Cell Line
  • Brain Neoplasms
  • Antibody Formation
  • Antibodies, Anti-Idiotypic
 

Citation

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Chicago
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MLA
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Mahaley, M. S., Gillespie, G. Y., Gillespie, R. P., Watkins, P. J., Bigner, D. D., Wikstrand, C. J., … Sanfilippo, F. (1983). Immunobiology of primary intracranial tumors. Part 8: Serological responses to active immunization of patients with anaplastic gliomas. J Neurosurg, 59(2), 208–216. https://doi.org/10.3171/jns.1983.59.2.0208
Mahaley, M. S., G. Y. Gillespie, R. P. Gillespie, P. J. Watkins, D. D. Bigner, C. J. Wikstrand, J. M. MacQueen, and F. Sanfilippo. “Immunobiology of primary intracranial tumors. Part 8: Serological responses to active immunization of patients with anaplastic gliomas.J Neurosurg 59, no. 2 (August 1983): 208–16. https://doi.org/10.3171/jns.1983.59.2.0208.
Mahaley MS, Gillespie GY, Gillespie RP, Watkins PJ, Bigner DD, Wikstrand CJ, et al. Immunobiology of primary intracranial tumors. Part 8: Serological responses to active immunization of patients with anaplastic gliomas. J Neurosurg. 1983 Aug;59(2):208–16.
Mahaley, M. S., et al. “Immunobiology of primary intracranial tumors. Part 8: Serological responses to active immunization of patients with anaplastic gliomas.J Neurosurg, vol. 59, no. 2, Aug. 1983, pp. 208–16. Pubmed, doi:10.3171/jns.1983.59.2.0208.
Mahaley MS, Gillespie GY, Gillespie RP, Watkins PJ, Bigner DD, Wikstrand CJ, MacQueen JM, Sanfilippo F. Immunobiology of primary intracranial tumors. Part 8: Serological responses to active immunization of patients with anaplastic gliomas. J Neurosurg. 1983 Aug;59(2):208–216.

Published In

J Neurosurg

DOI

ISSN

0022-3085

Publication Date

August 1983

Volume

59

Issue

2

Start / End Page

208 / 216

Location

United States

Related Subject Headings

  • Neurology & Neurosurgery
  • Lymphocytes
  • Immunization
  • Humans
  • HLA Antigens
  • Glioma
  • Cell Line
  • Brain Neoplasms
  • Antibody Formation
  • Antibodies, Anti-Idiotypic