Glial fibrillary acidic protein synthesized in vitro using messenger RNA from a human glioma cell line.

Published

Journal Article

Messenger RNA (mRNA) extracted from a continuous human glioma cell line grown in culture or as a solid tumor was translated in an mRNA-dependent reticulocyte lysate system. Translation products labeled with [35S]methionine were immunoprecipitated with antiserum specific for glial fibrillary acidic (GFA) protein, separated by one- and two-dimensional polyacrylamide gel electrophoresis and analyzed fluorographically. Immunoprecipitates from both cell culture and tumor mRNA translations had a molecular weight of 49,000 daltons, consistent with GFA protein extracted from human tissue. In two dimensions, the 49,000-dalton band resolved into two to three spots at pH 5.7-5.9, the isoelectric point of GFA protein. Minor lower molecular weight products were detected in fluorographs of heavily overloaded gels or in film exposed for extended periods of time. These data indicate that the GFA protein produced by this glioma cell line is chemically and immunologically similar to normal human GFA protein, which suggests that the primary phenotypic expression of GFA protein in this tumor cell line is not altered by the neoplastic process.

Full Text

Duke Authors

Cited Authors

  • Bigbee, JW; Bigner, DD; Eng, LF

Published Date

  • January 1, 1983

Published In

Volume / Issue

  • 42 / 1

Start / End Page

  • 80 - 86

PubMed ID

  • 6822846

Pubmed Central ID

  • 6822846

International Standard Serial Number (ISSN)

  • 0022-3069

Digital Object Identifier (DOI)

  • 10.1097/00005072-198301000-00007

Language

  • eng

Conference Location

  • England