A new ganglioside of the lactotetraose series, GalNAc-3'-isoLM1, detected in human meconium.
A monoclonal antibody produced by immunization with cells of the human glioma cell line D-54 MG reacted with ganglioside GM2. The binding epitope of the antibody was found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal. Immunological detection of glycolipid antigens on thin-layer plates with this monoclonal antibody, DMAb-1, revealed the presence of a new ganglioside. This ganglioside, co-migrating with NeuAc alpha 2-6Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer(6'-LM1) and GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GalNAc beta 1-4Gla beta 1-4Glc beta 1-1Cer (GalNAc-isoGM1) at chromatographic separation was isolated from human meconium. Its structure was determined by permethylation and fast atom bombardment-mass spectometry analyses. The new ganglioside was found to be a combination of the lacto and ganglio series gangliosides, and the structure found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GlcNAc alpha 1-3Gal beta 1-4Glc beta 1-1Cer(GalNAc-3'-isoLM1).
Duke Scholars
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Related Subject Headings
- Molecular Sequence Data
- Meconium
- Mass Spectrometry
- Infant, Newborn
- Humans
- Glycolipids
- Gangliosides
- G(M2) Ganglioside
- Epitopes
- Carbohydrate Sequence
Citation
Published In
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Molecular Sequence Data
- Meconium
- Mass Spectrometry
- Infant, Newborn
- Humans
- Glycolipids
- Gangliosides
- G(M2) Ganglioside
- Epitopes
- Carbohydrate Sequence