A new ganglioside of the lactotetraose series, GalNAc-3'-isoLM1, detected in human meconium.

Journal Article (Journal Article)

A monoclonal antibody produced by immunization with cells of the human glioma cell line D-54 MG reacted with ganglioside GM2. The binding epitope of the antibody was found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal. Immunological detection of glycolipid antigens on thin-layer plates with this monoclonal antibody, DMAb-1, revealed the presence of a new ganglioside. This ganglioside, co-migrating with NeuAc alpha 2-6Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer(6'-LM1) and GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GalNAc beta 1-4Gla beta 1-4Glc beta 1-1Cer (GalNAc-isoGM1) at chromatographic separation was isolated from human meconium. Its structure was determined by permethylation and fast atom bombardment-mass spectometry analyses. The new ganglioside was found to be a combination of the lacto and ganglio series gangliosides, and the structure found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GlcNAc alpha 1-3Gal beta 1-4Glc beta 1-1Cer(GalNAc-3'-isoLM1).

Full Text

Duke Authors

Cited Authors

  • Fredman, P; Månsson, JE; Wikstrand, CJ; Vrionis, FD; Rynmark, BM; Bigner, DD; Svennerholm, L

Published Date

  • July 25, 1989

Published In

Volume / Issue

  • 264 / 21

Start / End Page

  • 12122 - 12125

PubMed ID

  • 2473071

International Standard Serial Number (ISSN)

  • 0021-9258


  • eng

Conference Location

  • United States