Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification.


Journal Article

Primary malignant gliomas from 63 patients were analyzed to determine the relationship between amplification of the gene encoding the epidermal growth factor receptor (EGFR) and expression of the corresponding mRNA. Twenty-four tumors were found to have amplified the EGFR gene and amplification of other genes occurred in three additional tumors. Hybridization with synthetic RNA probes was used to quantitate mRNA levels in situ. All 24 tumors with amplification of the EGFR gene had high levels of expression of this gene, while none of the 39 tumors without amplification had increased levels. This shows that, in human gliomas, large increases in the expression of the EGFR gene are invariably associated with alterations in gene structure.

Full Text

Duke Authors

Cited Authors

  • Wong, AJ; Bigner, SH; Bigner, DD; Kinzler, KW; Hamilton, SR; Vogelstein, B

Published Date

  • October 1, 1987

Published In

Volume / Issue

  • 84 / 19

Start / End Page

  • 6899 - 6903

PubMed ID

  • 3477813

Pubmed Central ID

  • 3477813

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.84.19.6899


  • eng

Conference Location

  • United States