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Identifying potential tumor markers and antigens by database mining and rapid expression screening.

Publication ,  Journal Article
Loging, WT; Lal, A; Siu, IM; Loney, TL; Wikstrand, CJ; Marra, MA; Prange, C; Bigner, DD; Strausberg, RL; Riggins, GJ
Published in: Genome Res
September 2000

Genes expressed specifically in malignant tissue may have potential as therapeutic targets but have been difficult to locate for most cancers. The information hidden within certain public databases can reveal RNA transcripts specifically expressed in transformed tissue. To be useful, database information must be verified and a more complete pattern of tissue expression must be demonstrated. We tested database mining plus rapid screening by fluorescent-PCR expression comparison (F-PEC) as an approach to locate candidate brain tumor antigens. Cancer Genome Anatomy Project (CGAP) data was mined for genes highly expressed in glioblastoma multiforme. From 13 mined genes, seven showed potential as possible tumor markers or antigens as determined by further expression profiling. Now that large-scale expression information is readily available for many of the commonly occurring cancers, other candidate tumor markers or antigens could be located and evaluated with this approach.

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Published In

Genome Res

DOI

ISSN

1088-9051

Publication Date

September 2000

Volume

10

Issue

9

Start / End Page

1393 / 1402

Location

United States

Related Subject Headings

  • Reproducibility of Results
  • Polymerase Chain Reaction
  • Humans
  • Glioblastoma
  • Gene Expression Profiling
  • Fluorescent Dyes
  • Databases, Factual
  • Blotting, Western
  • Biomarkers, Tumor
  • Bioinformatics
 

Citation

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Loging, W. T., Lal, A., Siu, I. M., Loney, T. L., Wikstrand, C. J., Marra, M. A., … Riggins, G. J. (2000). Identifying potential tumor markers and antigens by database mining and rapid expression screening. Genome Res, 10(9), 1393–1402. https://doi.org/10.1101/gr.138000
Loging, W. T., A. Lal, I. M. Siu, T. L. Loney, C. J. Wikstrand, M. A. Marra, C. Prange, D. D. Bigner, R. L. Strausberg, and G. J. Riggins. “Identifying potential tumor markers and antigens by database mining and rapid expression screening.Genome Res 10, no. 9 (September 2000): 1393–1402. https://doi.org/10.1101/gr.138000.
Loging WT, Lal A, Siu IM, Loney TL, Wikstrand CJ, Marra MA, et al. Identifying potential tumor markers and antigens by database mining and rapid expression screening. Genome Res. 2000 Sep;10(9):1393–402.
Loging, W. T., et al. “Identifying potential tumor markers and antigens by database mining and rapid expression screening.Genome Res, vol. 10, no. 9, Sept. 2000, pp. 1393–402. Pubmed, doi:10.1101/gr.138000.
Loging WT, Lal A, Siu IM, Loney TL, Wikstrand CJ, Marra MA, Prange C, Bigner DD, Strausberg RL, Riggins GJ. Identifying potential tumor markers and antigens by database mining and rapid expression screening. Genome Res. 2000 Sep;10(9):1393–1402.

Published In

Genome Res

DOI

ISSN

1088-9051

Publication Date

September 2000

Volume

10

Issue

9

Start / End Page

1393 / 1402

Location

United States

Related Subject Headings

  • Reproducibility of Results
  • Polymerase Chain Reaction
  • Humans
  • Glioblastoma
  • Gene Expression Profiling
  • Fluorescent Dyes
  • Databases, Factual
  • Blotting, Western
  • Biomarkers, Tumor
  • Bioinformatics