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Immunohistochemical study of glial fibrillary acidic protein in avian sarcoma virus-induced gliomas in dogs.

Publication ,  Journal Article
Britt, RH; Lyons, BE; Eng, LF; Bigner, SH; Bigner, DD
Published in: J Neurooncol
1985

Anaplastic astrocytomas, gliosarcomas and sarcomas were induced in 33 neonatal dogs by intracerebral injection of purified Schmidt-Ruppin strain of avian sarcoma virus (ASV). A total of 11 anaplastic gliomas, 7 gliosarcomas and 18 sarcomas were induced. Ten of the 11 anaplastic astrocytomas and all 7 gliosarcomas were positive for GFAP using the peroxidase-antiperoxidase method on formalin-fixed, paraffin embedded tissue. None of the sarcomas were positive for GFAP. One anaplastic astrocytoma was analyzed by gel electrophoresis and immunoblot and the amount of GFAP in the tumor was compared with comparable samples from normal white and gray matter. Densitometric analysis of the GFAP stained gels showed that 12-13% of tumor protein was GFAP compared with 2-3% for the non-neoplastic white and gray matter. The results of this study add further evidence to the astrocytic origin of many of the induced anaplastic primary brain tumors by ASV.

Duke Scholars

Published In

J Neurooncol

DOI

ISSN

0167-594X

Publication Date

1985

Volume

3

Issue

1

Start / End Page

53 / 59

Location

United States

Related Subject Headings

  • Tomography, X-Ray Computed
  • Sarcoma, Experimental
  • Oncology & Carcinogenesis
  • Neoplasms, Multiple Primary
  • Immunoenzyme Techniques
  • Glioma
  • Glial Fibrillary Acidic Protein
  • Dogs
  • Brain Neoplasms
  • Brain
 

Citation

APA
Chicago
ICMJE
MLA
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Britt, R. H., Lyons, B. E., Eng, L. F., Bigner, S. H., & Bigner, D. D. (1985). Immunohistochemical study of glial fibrillary acidic protein in avian sarcoma virus-induced gliomas in dogs. J Neurooncol, 3(1), 53–59. https://doi.org/10.1007/BF00165172
Britt, R. H., B. E. Lyons, L. F. Eng, S. H. Bigner, and D. D. Bigner. “Immunohistochemical study of glial fibrillary acidic protein in avian sarcoma virus-induced gliomas in dogs.J Neurooncol 3, no. 1 (1985): 53–59. https://doi.org/10.1007/BF00165172.
Britt RH, Lyons BE, Eng LF, Bigner SH, Bigner DD. Immunohistochemical study of glial fibrillary acidic protein in avian sarcoma virus-induced gliomas in dogs. J Neurooncol. 1985;3(1):53–9.
Britt, R. H., et al. “Immunohistochemical study of glial fibrillary acidic protein in avian sarcoma virus-induced gliomas in dogs.J Neurooncol, vol. 3, no. 1, 1985, pp. 53–59. Pubmed, doi:10.1007/BF00165172.
Britt RH, Lyons BE, Eng LF, Bigner SH, Bigner DD. Immunohistochemical study of glial fibrillary acidic protein in avian sarcoma virus-induced gliomas in dogs. J Neurooncol. 1985;3(1):53–59.
Journal cover image

Published In

J Neurooncol

DOI

ISSN

0167-594X

Publication Date

1985

Volume

3

Issue

1

Start / End Page

53 / 59

Location

United States

Related Subject Headings

  • Tomography, X-Ray Computed
  • Sarcoma, Experimental
  • Oncology & Carcinogenesis
  • Neoplasms, Multiple Primary
  • Immunoenzyme Techniques
  • Glioma
  • Glial Fibrillary Acidic Protein
  • Dogs
  • Brain Neoplasms
  • Brain