Hydroxyurea synchronization increases mitotic yield in human glioma cell lines.
Journal Article (Journal Article)
Karyotypic studies of human gliomas are often limited by a low mitotic index and the appearance of contracted chromosomes that do not exhibit clear banding patterns. The purpose of this study was to investigate the use of hydroxyurea (HU) as a synchronizing agent using established human glioma cell lines as a model system. HU was shown to reversibly inhibit cellular replication in glioma cell lines U-251MG, D-245MG, D-247MG and D-263MG by flow cytometry on the basis of DNA content. Two-to sixfold increases were demonstrated in the mitotic index of HU-treated cultures exhibiting the greatest percentage of cells in the G2/M phases of the cell cycle. HU, therefore, promises to be an effective agent for use in short term cultures from biopsied human tumors to increase the quality of chromosome preparations in these tumors.
Full Text
Duke Authors
Cited Authors
- Biegel, JA; Leslie, DS; Bigner, DD; Bigner, SH
Published Date
- 1987
Published In
Volume / Issue
- 73 / 3
Start / End Page
- 309 - 312
PubMed ID
- 3618122
International Standard Serial Number (ISSN)
- 0001-6322
Digital Object Identifier (DOI)
- 10.1007/BF00686628
Language
- eng
Conference Location
- Germany