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Anti-GM2 monoclonal antibodies induce necrosis in GM2-rich cultures of a human glioma cell line.

Publication ,  Journal Article
Bjerkvig, R; Engebraaten, O; Laerum, OD; Fredman, P; Svennerholm, L; Vrionis, FD; Wikstrand, CJ; Bigner, DD
Published in: Cancer Res
September 1, 1991

The effects of four anti-GM2 monoclonal antibodies (DMAb-1, DMAb-2, DMAb-3, and DMAb-5) were studied on spheroid cultures from a human glioma cell line (D-54 MG) that is known to express high levels of GM2. The spheroids developed central necrosis 48 h after antibody exposures at concentrations greater than 6 micrograms/ml. No necrosis was found with antibodies that had been absorbed with GM2 prior to exposure or with unrelated cytotoxic antibodies. Immunohistochemistry showed that the necrosis started shortly after the antibodies were evenly distributed throughout the spheroids. Light and transmission electron microscopy revealed that a small portion of the cells, mainly in the periphery of the spheroids, was unaffected by antibody exposure. New monolayer cultures established from antibody-treated cells expressed a 50% lower GM2 content as shown by flow cytometry and determination of ganglioside content throughout at least 12 passages. Thus, the GM2-rich D-54 MG cell line has subpopulations of cells with lower GM2 content. Spheroids obtained from this subpopulation developed only minor necrosis after antibody treatment. These results show that GM2 antibodies cause severe necrosis of GM2-containing glioma cells in vitro, but the effect depends on the concentration of antigen, and a threshold number of GM2 molecules is required.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

September 1, 1991

Volume

51

Issue

17

Start / End Page

4643 / 4648

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Necrosis
  • Microscopy, Fluorescence
  • Microscopy, Electron
  • Humans
  • Glioma
  • G(M2) Ganglioside
  • Cell Survival
  • Antibodies, Monoclonal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bjerkvig, R., Engebraaten, O., Laerum, O. D., Fredman, P., Svennerholm, L., Vrionis, F. D., … Bigner, D. D. (1991). Anti-GM2 monoclonal antibodies induce necrosis in GM2-rich cultures of a human glioma cell line. Cancer Res, 51(17), 4643–4648.
Bjerkvig, R., O. Engebraaten, O. D. Laerum, P. Fredman, L. Svennerholm, F. D. Vrionis, C. J. Wikstrand, and D. D. Bigner. “Anti-GM2 monoclonal antibodies induce necrosis in GM2-rich cultures of a human glioma cell line.Cancer Res 51, no. 17 (September 1, 1991): 4643–48.
Bjerkvig R, Engebraaten O, Laerum OD, Fredman P, Svennerholm L, Vrionis FD, et al. Anti-GM2 monoclonal antibodies induce necrosis in GM2-rich cultures of a human glioma cell line. Cancer Res. 1991 Sep 1;51(17):4643–8.
Bjerkvig, R., et al. “Anti-GM2 monoclonal antibodies induce necrosis in GM2-rich cultures of a human glioma cell line.Cancer Res, vol. 51, no. 17, Sept. 1991, pp. 4643–48.
Bjerkvig R, Engebraaten O, Laerum OD, Fredman P, Svennerholm L, Vrionis FD, Wikstrand CJ, Bigner DD. Anti-GM2 monoclonal antibodies induce necrosis in GM2-rich cultures of a human glioma cell line. Cancer Res. 1991 Sep 1;51(17):4643–4648.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

September 1, 1991

Volume

51

Issue

17

Start / End Page

4643 / 4648

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Necrosis
  • Microscopy, Fluorescence
  • Microscopy, Electron
  • Humans
  • Glioma
  • G(M2) Ganglioside
  • Cell Survival
  • Antibodies, Monoclonal