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Expression of murine leukemia virus structural antigens on the surface of chemically induced murine sarcomas.

Publication ,  Journal Article
Grant, JP; Bigner, DD; Fischinger, PJ; Bolognesi, DP
Published in: Proc Natl Acad Sci U S A
December 1974
Published version (DOI) Link to item

Cultured cells of different chemically-induced C57BL/6N murine sarcomas produced variable amounts of infectious murine leukemia virus (MuLV) and contained proportional amounts of MuLV structural components as determined by radioimmunoassay. Monospecific antisera directed against the major MuLV glycoprotein (gp71), the major internal antigen (p30), and the ribonucleoprotein (p10) were capable of mediating tumor cell lysis in the presence of complement, suggesting that these viral structural components were localized at least in part to the cell surface. Membrane immunofluorescence studies with MuLV p30 antiserum confirmed surface localization. Addition of MuLV p30 polypeptide to normal cells and tumor cells enhanced the cytotoxicity of MuLV p30 antiserum. Studies are presented which suggest that the presence of MuLV structural components on cell surfaces can be independent of virus production and cellular transformation.

Duke Scholars

John Palmer Grant
Author John Palmer Grant Surgery, Minimally Invasive Surgery
Darell Doty Bigner
Author Darell Doty Bigner Neurosurgery
Dani Paul Bolognesi
Author Dani Paul Bolognesi Surgery, Surgical Sciences

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.71.12.5037

ISSN

0027-8424

Publication Date

December 1974

Volume

71

Issue

12

Start / End Page

5037 / 5041

Location

United States

Related Subject Headings

  • Sarcoma, Experimental
  • Radioimmunoassay
  • Peptides
  • Mice
  • Methylcholanthrene
  • Leukemia Virus, Murine
  • Fibroblasts
  • Cytotoxicity Tests, Immunologic
  • Complement System Proteins
  • Cell Membrane
 

Citation

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Grant, J. P., Bigner, D. D., Fischinger, P. J., & Bolognesi, D. P. (1974). Expression of murine leukemia virus structural antigens on the surface of chemically induced murine sarcomas. Proc Natl Acad Sci U S A, 71(12), 5037–5041. https://doi.org/10.1073/pnas.71.12.5037
Grant, J. P., D. D. Bigner, P. J. Fischinger, and D. P. Bolognesi. “Expression of murine leukemia virus structural antigens on the surface of chemically induced murine sarcomas.” Proc Natl Acad Sci U S A 71, no. 12 (December 1974): 5037–41. https://doi.org/10.1073/pnas.71.12.5037.
Grant JP, Bigner DD, Fischinger PJ, Bolognesi DP. Expression of murine leukemia virus structural antigens on the surface of chemically induced murine sarcomas. Proc Natl Acad Sci U S A. 1974 Dec;71(12):5037–41.
Grant, J. P., et al. “Expression of murine leukemia virus structural antigens on the surface of chemically induced murine sarcomas.” Proc Natl Acad Sci U S A, vol. 71, no. 12, Dec. 1974, pp. 5037–41. Pubmed, doi:10.1073/pnas.71.12.5037.
Grant JP, Bigner DD, Fischinger PJ, Bolognesi DP. Expression of murine leukemia virus structural antigens on the surface of chemically induced murine sarcomas. Proc Natl Acad Sci U S A. 1974 Dec;71(12):5037–5041.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.71.12.5037

ISSN

0027-8424

Publication Date

December 1974

Volume

71

Issue

12

Start / End Page

5037 / 5041

Location

United States

Related Subject Headings

  • Sarcoma, Experimental
  • Radioimmunoassay
  • Peptides
  • Mice
  • Methylcholanthrene
  • Leukemia Virus, Murine
  • Fibroblasts
  • Cytotoxicity Tests, Immunologic
  • Complement System Proteins
  • Cell Membrane