Characterization of new gangliosides of the lactotetraose series in murine xenografts of a human glioma cell line.

Published

Journal Article

The major mono- and disialogangliosides of the extensively characterized established human glioma line D54MG were isolated and purified from subcutaneous solid xenografts grown in athymic (nu/nu) mice. Structural determination showed that they belonged to the lactotetraosylceramide series. The sialyllactotetraosylceramide contained 90% N-glycolyl- and 10% N-acetylneuraminic acid linked in an alpha 2-3 linkage (IV3NeuGc-LcOse4Cer, IV3NeuAc-LcOse4Cer). The disialogangliosides had a previously undescribed type of structure with sialic acids linked to the terminal galactose in an alpha 2-3 linkage and to N-acetylglucosamine in an alpha 2-6 linkage. Not only did species with NeuAc or NeuGc occur, but also species with mixtures of the two sialic acids, e.g. NeuAc and NeuGc. The schematic structures of the new disialogangliosides are (Formula:see text).

Full Text

Duke Authors

Cited Authors

  • Månsson, JE; Fredman, P; Bigner, DD; Molin, K; Rosengren, B; Friedman, HS; Svennerholm, L

Published Date

  • May 1, 1986

Published In

Volume / Issue

  • 201 / 1

Start / End Page

  • 109 - 113

PubMed ID

  • 3011500

Pubmed Central ID

  • 3011500

Electronic International Standard Serial Number (EISSN)

  • 1873-3468

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/0014-5793(86)80580-4

Language

  • eng