Expression of MXI1, a Myc antagonist, is regulated by Sp1 and AP2.

Published

Journal Article

MXI1, a member of the MAD family of Myc antagonists, encodes a transcription factor whose expression must be tightly regulated to maintain normal cell growth and differentiation. To more closely investigate the transcriptional regulation of the human MXI1 gene, we have cloned and characterized the MXI1 promoter. After clarification of the 5'- and 3'-untranslated regions of the cDNA (indicating that the true length of the MXI1 transcript is 2643 base pairs), we identified two transcription initiation sites. We subsequently isolated the MXI1 promoter, which is GC-rich and lacks a TATA box. Although it contains at least six potential initiator sequences, functional studies indicate the proximal two initiator sequences in combination with nearby Sp1 and MED-1 sites together account for virtually all promoter activity. We also demonstrate that MXI1 promoter activity is repressed by high levels of AP2. These studies provide further insight into the complex regulatory mechanisms governing MXI1 gene expression and its role in cellular differentiation and tumor suppression.

Full Text

Cited Authors

  • Benson, LQ; Coon, MR; Krueger, LM; Han, GC; Sarnaik, AA; Wechsler, DS

Published Date

  • October 1999

Published In

Volume / Issue

  • 274 / 40

Start / End Page

  • 28794 - 28802

PubMed ID

  • 10497252

Pubmed Central ID

  • 10497252

Electronic International Standard Serial Number (EISSN)

  • 1083-351X

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.274.40.28794

Language

  • eng