Interleukin-1 stimulates diacylglycerol production in T lymphocytes by a novel mechanism.

Published

Journal Article

We have investigated the biochemical mechanism by which interleukin-1 (IL-1) serves as a comitogen with agents that directly activate the antigen receptor in T lymphocytes. We have studied the human T cell line Jurkat, which can be stimulated to produce Interleukin-2 by treatment with antibodies that bind to the CD3-antigen receptor complex and hence represents a model system for T cell activation. Using highly purified, recombinant human IL-1, we show that IL-1 stimulates rapid diacylglycerol and phosphorylcholine production from phosphatidylcholine (PC) in the absence of phosphatidylinositol turnover in Jurkat cells. This effect is also observed in peripheral blood T cells and a murine T cell line. The EC50 for IL-1 was 28 fM, and PC hydrolysis was detectable within 5 sec at 37 degrees C. The murine cell line had typical high-affinity IL-1 receptors (kd = 7 X 10(-11) M). However, we were unable to detect IL-1 binding to Jurkat cells. This reaction occurs via a novel mechanism and may explain the comitogenic activity of IL-1 in T lymphocyte activation as well as many of the pleiotropic biologic effects of this cytokine.

Full Text

Duke Authors

Cited Authors

  • Rosoff, PM; Savage, N; Dinarello, CA

Published Date

  • July 1, 1988

Published In

Volume / Issue

  • 54 / 1

Start / End Page

  • 73 - 81

PubMed ID

  • 2898301

Pubmed Central ID

  • 2898301

International Standard Serial Number (ISSN)

  • 0092-8674

Language

  • eng

Conference Location

  • United States