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Identification of a 43-kilodalton human T lymphocyte membrane protein as a receptor for pertussis toxin.

Publication ,  Journal Article
Rogers, TS; Corey, SJ; Rosoff, PM
Published in: J Immunol
July 15, 1990

Pertussis toxin (PTx), an exotoxin of Bordetella pertussis has been used as a molecular probe to study stimulus-response coupling in a wide variety of cells. We have previously shown that PTx activates the same signal transduction pathways as Ag or mAb directed against the CD3-T cell Ag receptor complex in human T cells. Because the EC50 for mitogenic stimulation by PTx was 1.7 nM, we suspected that the toxin was specifically interacting with a membrane protein or receptor. We have used both chemical cross-linking and Western blotting techniques to demonstrate that PTx shows specific binding to a 43 kDa-membrane protein on cells that respond to PTx by rapid second messenger production. The PTx receptor can be detected in both the E6-1 Jurkat cell line and a CD3-TCR-negative Jurkat line, demonstrating that it is not coordinately expressed with the Ag receptor complex. The 43 kDa-protein is also found in the HPB-ALL human T cell line and PBL, but not in a murine T cell hybridoma or human neutrophils, both of which are unresponsive to PTx activation. These data suggest that the biochemical basis for the mitogenic activity of PTx may lie in its binding to a specific membrane receptor that is capable of transmitting an activation signal.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1990

Volume

145

Issue

2

Start / End Page

678 / 683

Location

United States

Related Subject Headings

  • Virulence Factors, Bordetella
  • T-Lymphocytes
  • Signal Transduction
  • Receptors, Cell Surface
  • Pertussis Toxin
  • Molecular Weight
  • Membrane Proteins
  • Immunology
  • Humans
  • GTP-Binding Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Rogers, T. S., Corey, S. J., & Rosoff, P. M. (1990). Identification of a 43-kilodalton human T lymphocyte membrane protein as a receptor for pertussis toxin. J Immunol, 145(2), 678–683.
Rogers, T. S., S. J. Corey, and P. M. Rosoff. “Identification of a 43-kilodalton human T lymphocyte membrane protein as a receptor for pertussis toxin.J Immunol 145, no. 2 (July 15, 1990): 678–83.
Rogers TS, Corey SJ, Rosoff PM. Identification of a 43-kilodalton human T lymphocyte membrane protein as a receptor for pertussis toxin. J Immunol. 1990 Jul 15;145(2):678–83.
Rogers, T. S., et al. “Identification of a 43-kilodalton human T lymphocyte membrane protein as a receptor for pertussis toxin.J Immunol, vol. 145, no. 2, July 1990, pp. 678–83.
Rogers TS, Corey SJ, Rosoff PM. Identification of a 43-kilodalton human T lymphocyte membrane protein as a receptor for pertussis toxin. J Immunol. 1990 Jul 15;145(2):678–683.

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1990

Volume

145

Issue

2

Start / End Page

678 / 683

Location

United States

Related Subject Headings

  • Virulence Factors, Bordetella
  • T-Lymphocytes
  • Signal Transduction
  • Receptors, Cell Surface
  • Pertussis Toxin
  • Molecular Weight
  • Membrane Proteins
  • Immunology
  • Humans
  • GTP-Binding Proteins