Reconstitution of a T cell receptor-stimulated plasma membrane calcium transporter: lack of dependence on inositol phosphates.

Published

Journal Article

The activation of T lymphocytes, like many cells, requires a rapid rise in intracellular Ca2+ secondary to both an influx and a release from intracellular stores. The latter is activated by inositol-1,4,5-trisphosphate [Ins(1,4,5)P3]. It is controversial if inositol phosphates can also stimulate a plasma membrane Ca2+ channel in T cells. We have studied the human T cell line HPB-ALL which, upon stimulation of its antigen receptor, does not generate detectable levels of Ins(1,4,5)P3 or internal Ca2+ release, but does have a Ca2+ influx. We have reconstituted a receptor-activated Ca2+ transporter from plasma membranes from these cells which has properties similar to the transporter observed in vivo and does not require inositol phosphates for activation. These data show that mitogens may activate more than one type of ligand-gated Ca2+ transport mechanism in T lymphocytes.

Full Text

Duke Authors

Cited Authors

  • O'Riordan, C; Rosoff, PM

Published Date

  • February 1993

Published In

Volume / Issue

  • 14 / 2

Start / End Page

  • 119 - 133

PubMed ID

  • 8384530

Pubmed Central ID

  • 8384530

International Standard Serial Number (ISSN)

  • 0143-4160

Language

  • eng

Conference Location

  • Netherlands