Skip to main content
Journal cover image

Reconstitution of a T cell receptor-stimulated plasma membrane calcium transporter: lack of dependence on inositol phosphates.

Publication ,  Journal Article
O'Riordan, C; Rosoff, PM
Published in: Cell Calcium
February 1993

The activation of T lymphocytes, like many cells, requires a rapid rise in intracellular Ca2+ secondary to both an influx and a release from intracellular stores. The latter is activated by inositol-1,4,5-trisphosphate [Ins(1,4,5)P3]. It is controversial if inositol phosphates can also stimulate a plasma membrane Ca2+ channel in T cells. We have studied the human T cell line HPB-ALL which, upon stimulation of its antigen receptor, does not generate detectable levels of Ins(1,4,5)P3 or internal Ca2+ release, but does have a Ca2+ influx. We have reconstituted a receptor-activated Ca2+ transporter from plasma membranes from these cells which has properties similar to the transporter observed in vivo and does not require inositol phosphates for activation. These data show that mitogens may activate more than one type of ligand-gated Ca2+ transport mechanism in T lymphocytes.

Duke Scholars

Published In

Cell Calcium

DOI

ISSN

0143-4160

Publication Date

February 1993

Volume

14

Issue

2

Start / End Page

119 / 133

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes
  • Signal Transduction
  • Receptors, Antigen, T-Cell
  • Lymphocyte Activation
  • Inositol 1,4,5-Trisphosphate
  • Humans
  • Cell Membrane
  • Calcium-Transporting ATPases
  • Calcium Channels
 

Citation

APA
Chicago
ICMJE
MLA
NLM
O’Riordan, C., & Rosoff, P. M. (1993). Reconstitution of a T cell receptor-stimulated plasma membrane calcium transporter: lack of dependence on inositol phosphates. Cell Calcium, 14(2), 119–133. https://doi.org/10.1016/0143-4160(93)90082-h
O’Riordan, C., and P. M. Rosoff. “Reconstitution of a T cell receptor-stimulated plasma membrane calcium transporter: lack of dependence on inositol phosphates.Cell Calcium 14, no. 2 (February 1993): 119–33. https://doi.org/10.1016/0143-4160(93)90082-h.
O’Riordan, C., and P. M. Rosoff. “Reconstitution of a T cell receptor-stimulated plasma membrane calcium transporter: lack of dependence on inositol phosphates.Cell Calcium, vol. 14, no. 2, Feb. 1993, pp. 119–33. Pubmed, doi:10.1016/0143-4160(93)90082-h.
Journal cover image

Published In

Cell Calcium

DOI

ISSN

0143-4160

Publication Date

February 1993

Volume

14

Issue

2

Start / End Page

119 / 133

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes
  • Signal Transduction
  • Receptors, Antigen, T-Cell
  • Lymphocyte Activation
  • Inositol 1,4,5-Trisphosphate
  • Humans
  • Cell Membrane
  • Calcium-Transporting ATPases
  • Calcium Channels