Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice.

Journal Article (Journal Article)

Conflicting evidence supports a role for tau as an essential neuronal cytoskeletal protein or as a redundant protein whose function can be fulfilled by other microtubule-associated proteins. To investigate the function of tau in axonogenesis, we created tau deficient mice by disrupting the TAU gene. The engineered mice do not express the tau protein, appear physically normal and are able to reproduce. In contrast to a previously reported tau knockout mouse, embryonic hippocampal cultures from tau deficient mice show a significant delay in maturation as measured by axonal and neuritic extensions. The classic technique of selectively enhancing axonal growth by growth on laminin substrates failed to restore normal neuronal maturation of tau knockout neurons. By mating human TAU-gene transgenic and tau knockout mice, we reconstituted tau-deficient neurons with human tau proteins and restored a normal pattern of axonal growth and neuronal maturation. The ability of human tau proteins to rescue tau-deficient mouse neurons confirms that tau expression affects the rate of neurite extension.

Full Text

Duke Authors

Cited Authors

  • Dawson, HN; Ferreira, A; Eyster, MV; Ghoshal, N; Binder, LI; Vitek, MP

Published Date

  • March 2001

Published In

Volume / Issue

  • 114 / Pt 6

Start / End Page

  • 1179 - 1187

PubMed ID

  • 11228161

International Standard Serial Number (ISSN)

  • 0021-9533

Digital Object Identifier (DOI)

  • 10.1242/jcs.114.6.1179


  • eng

Conference Location

  • England