Triplet repeats form secondary structures that escape DNA repair in yeast.

Published

Journal Article

Several human neurodegenerative diseases result from expansion of CTG/CAG or CGG/CCG triplet repeats. The finding that single-stranded CNG repeats form hairpin-like structures in vitro has led to the hypothesis that DNA secondary structure formation is an important component of the expansion mechanism. We show that single-stranded DNA loops containing 10 CTG/CAG or CGG/CCG repeats are inefficiently repaired during meiotic recombination in Saccharomyces cerevisiae. Comparisons of the repair of DNA loops with palindromic and nonpalindromic sequences suggest that this inefficient repair reflects the ability of these sequences to form hairpin structures in vivo.

Full Text

Duke Authors

Cited Authors

  • Moore, H; Greenwell, PW; Liu, CP; Arnheim, N; Petes, TD

Published Date

  • February 16, 1999

Published In

Volume / Issue

  • 96 / 4

Start / End Page

  • 1504 - 1509

PubMed ID

  • 9990053

Pubmed Central ID

  • 9990053

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.96.4.1504

Language

  • eng

Conference Location

  • United States