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Redox regulation of the rat hepatocyte iNOS promoter.

Publication ,  Journal Article
Punzalan, C; Cai, C; Schroeder, RA; Kuo, PC
Published in: Surgery
August 1999

BACKGROUND: The purpose of this study was to define the redox sensitive cis-acting transcriptional mechanisms that regulate inducible nitric oxide synthase (iNOS) promoter function in the hepatocyte. METHODS: Clonal deletion constructs of the rat hepatocyte iNOS promoter (Genbank X95629; 1845 base pair)-reporter plasmid were transiently transfected into HepG2 cells treated with IL-1 beta and IL-1 beta + hydrogen peroxide. RESULTS: A segment of the promoter upstream from nucleotide -1126 was associated with redox-sensitive augmentation of promoter activity. Site-directed mutagenesis of 2 antioxidant response elements (AREs) was combined with transfection analysis to demonstrate that mutation of the ARE at nt-1347 ablated oxidant stress-mediated activation of the iNOS promoter. CONCLUSIONS: This ARE conveys the redox-sensitive response of the rat iNOS promoter. Hepatocyte iNOS expression is a novel and, as yet, poorly described antioxidant mechanism that is cytokine and redox sensitive and that plays a pleuripotent regulatory role in hepatocellular function in the face of sepsis and shock.

Duke Scholars

Published In

Surgery

ISSN

0039-6060

Publication Date

August 1999

Volume

126

Issue

2

Start / End Page

450 / 455

Location

United States

Related Subject Headings

  • Surgery
  • Response Elements
  • Rats
  • Promoter Regions, Genetic
  • Oxidative Stress
  • Oxidation-Reduction
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase
  • Liver
  • Interleukin-1
 

Citation

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MLA
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Punzalan, C., Cai, C., Schroeder, R. A., & Kuo, P. C. (1999). Redox regulation of the rat hepatocyte iNOS promoter. Surgery, 126(2), 450–455.
Punzalan, C., C. Cai, R. A. Schroeder, and P. C. Kuo. “Redox regulation of the rat hepatocyte iNOS promoter.Surgery 126, no. 2 (August 1999): 450–55.
Punzalan C, Cai C, Schroeder RA, Kuo PC. Redox regulation of the rat hepatocyte iNOS promoter. Surgery. 1999 Aug;126(2):450–5.
Punzalan, C., et al. “Redox regulation of the rat hepatocyte iNOS promoter.Surgery, vol. 126, no. 2, Aug. 1999, pp. 450–55.
Punzalan C, Cai C, Schroeder RA, Kuo PC. Redox regulation of the rat hepatocyte iNOS promoter. Surgery. 1999 Aug;126(2):450–455.
Journal cover image

Published In

Surgery

ISSN

0039-6060

Publication Date

August 1999

Volume

126

Issue

2

Start / End Page

450 / 455

Location

United States

Related Subject Headings

  • Surgery
  • Response Elements
  • Rats
  • Promoter Regions, Genetic
  • Oxidative Stress
  • Oxidation-Reduction
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase
  • Liver
  • Interleukin-1