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Oxidative stress increases hepatocyte iNOS gene transcription and promoter activity.

Publication ,  Journal Article
Kuo, PC; Abe, KY; Schroeder, RA
Published in: Biochem Biophys Res Commun
May 19, 1997

Hepatocyte expression of inducible nitric oxide synthase (iNOS) is initiated by the presence of pro-inflammatory cytokines, such as interleukin-1beta (IL-1). In the presence of oxidative stress, IL-1beta mediated hepatocyte iNOS expression and NO synthesis are significantly increased. To determine the underlying molecular mechanism responsible for oxidative stress augmentation of hepatocyte iNOS expression, rat hepatocytes in primary culture were stimulated with IL-1beta (250 U/mL) in the presence and absence of benzenetriol (BZT, 0-100 microM), an autocatalytic source of superoxide at physiologic pH. Nuclear runon analysis demonstrated that BZT was associated with increased iNOS gene transcription in the setting of IL-1 stimulation. Transient transfection of a plasmid construct composed of the rat hepatocyte iNOS promoter and a chloramphenicol transferase reporter gene demonstrated that the combination of BZT and IL-1 significantly increased iNOS promoter activity in comparison to that of IL-1beta alone. These data indicate that BZT-mediated oxidative stress increases IL-1beta induced iNOS gene transcription and iNOS promoter activity.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

May 19, 1997

Volume

234

Issue

2

Start / End Page

289 / 292

Location

United States

Related Subject Headings

  • Transfection
  • Transcription, Genetic
  • Rats, Inbred Lew
  • Rats
  • Promoter Regions, Genetic
  • Oxidative Stress
  • Nitric Oxide Synthase
  • Male
  • Liver
  • Interleukin-1
 

Citation

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Kuo, P. C., Abe, K. Y., & Schroeder, R. A. (1997). Oxidative stress increases hepatocyte iNOS gene transcription and promoter activity. Biochem Biophys Res Commun, 234(2), 289–292. https://doi.org/10.1006/bbrc.1997.6562
Kuo, P. C., K. Y. Abe, and R. A. Schroeder. “Oxidative stress increases hepatocyte iNOS gene transcription and promoter activity.Biochem Biophys Res Commun 234, no. 2 (May 19, 1997): 289–92. https://doi.org/10.1006/bbrc.1997.6562.
Kuo PC, Abe KY, Schroeder RA. Oxidative stress increases hepatocyte iNOS gene transcription and promoter activity. Biochem Biophys Res Commun. 1997 May 19;234(2):289–92.
Kuo, P. C., et al. “Oxidative stress increases hepatocyte iNOS gene transcription and promoter activity.Biochem Biophys Res Commun, vol. 234, no. 2, May 1997, pp. 289–92. Pubmed, doi:10.1006/bbrc.1997.6562.
Kuo PC, Abe KY, Schroeder RA. Oxidative stress increases hepatocyte iNOS gene transcription and promoter activity. Biochem Biophys Res Commun. 1997 May 19;234(2):289–292.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

May 19, 1997

Volume

234

Issue

2

Start / End Page

289 / 292

Location

United States

Related Subject Headings

  • Transfection
  • Transcription, Genetic
  • Rats, Inbred Lew
  • Rats
  • Promoter Regions, Genetic
  • Oxidative Stress
  • Nitric Oxide Synthase
  • Male
  • Liver
  • Interleukin-1