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ZAP-70 tyrosine kinase is required for the up-regulation of Fas ligand in activation-induced T cell apoptosis.

Publication ,  Journal Article
Eischen, CM; Williams, BL; Zhang, W; Samelson, LE; Lynch, DH; Abraham, RT; Leibson, PJ
Published in: J Immunol
August 1, 1997

Activation-induced cell death (AICD) is initiated by the TCR-dependent up-regulation of Fas ligand (FasL) mRNA. The subsequently generated soluble or cell-associated FasL gene products bind Fas, leading to apoptosis of the T cells. Although TCR stimulation is essential to initiate AICD, little is known about which TCR-initiated second messengers are required for FasL expression. We provide evidence in this work that T cells lacking the tyrosine kinase ZAP-70 are unable to up-regulate FasL and undergo AICD. Transfection of wild-type ZAP-70 into the ZAP-70-deficient T cells restores their sensitivity to TCR-induced apoptosis, whereas transfection of catalytically inactive ZAP-70 does not. These results provide clear evidence that ZAP-70 tyrosine kinase is essential in up-regulating FasL for TCR-induced apoptosis.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

August 1, 1997

Volume

159

Issue

3

Start / End Page

1135 / 1139

Location

United States

Related Subject Headings

  • fas Receptor
  • ZAP-70 Protein-Tyrosine Kinase
  • Up-Regulation
  • T-Lymphocytes
  • Receptors, Antigen, T-Cell
  • RNA, Messenger
  • Protein-Tyrosine Kinases
  • Membrane Glycoproteins
  • Lymphocyte Activation
  • Ligands
 

Citation

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Eischen, C. M., Williams, B. L., Zhang, W., Samelson, L. E., Lynch, D. H., Abraham, R. T., & Leibson, P. J. (1997). ZAP-70 tyrosine kinase is required for the up-regulation of Fas ligand in activation-induced T cell apoptosis. J Immunol, 159(3), 1135–1139.
Eischen, C. M., B. L. Williams, W. Zhang, L. E. Samelson, D. H. Lynch, R. T. Abraham, and P. J. Leibson. “ZAP-70 tyrosine kinase is required for the up-regulation of Fas ligand in activation-induced T cell apoptosis.J Immunol 159, no. 3 (August 1, 1997): 1135–39.
Eischen CM, Williams BL, Zhang W, Samelson LE, Lynch DH, Abraham RT, et al. ZAP-70 tyrosine kinase is required for the up-regulation of Fas ligand in activation-induced T cell apoptosis. J Immunol. 1997 Aug 1;159(3):1135–9.
Eischen, C. M., et al. “ZAP-70 tyrosine kinase is required for the up-regulation of Fas ligand in activation-induced T cell apoptosis.J Immunol, vol. 159, no. 3, Aug. 1997, pp. 1135–39.
Eischen CM, Williams BL, Zhang W, Samelson LE, Lynch DH, Abraham RT, Leibson PJ. ZAP-70 tyrosine kinase is required for the up-regulation of Fas ligand in activation-induced T cell apoptosis. J Immunol. 1997 Aug 1;159(3):1135–1139.

Published In

J Immunol

ISSN

0022-1767

Publication Date

August 1, 1997

Volume

159

Issue

3

Start / End Page

1135 / 1139

Location

United States

Related Subject Headings

  • fas Receptor
  • ZAP-70 Protein-Tyrosine Kinase
  • Up-Regulation
  • T-Lymphocytes
  • Receptors, Antigen, T-Cell
  • RNA, Messenger
  • Protein-Tyrosine Kinases
  • Membrane Glycoproteins
  • Lymphocyte Activation
  • Ligands