Dynamic actin polymerization drives T cell receptor-induced spreading: a role for the signal transduction adaptor LAT.

Published

Journal Article

T cell activation induces functional changes in cell shape and cytoskeletal architecture. To facilitate the collection of dynamic, high-resolution images of activated T cells, we plated T cells on coverslips coated with antibodies to the T cell receptor (TCR). Using these images, we were able to quantitate the morphological responses of individual cells over time. Here, we show that TCR engagement triggers the formation and expansion of contacts bounded by continuously remodeled actin-rich rings. These processes are associated with the extension of lamellipodia and require actin polymerization, tyrosine kinase activation, cytoplasmic calcium increases, and LAT, an important hematopoietic adaptor. In addition, the maintenance of the resulting contact requires sustained calcium influxes, an intact microtubule cytoskeleton, and functional LAT.

Full Text

Duke Authors

Cited Authors

  • Bunnell, SC; Kapoor, V; Trible, RP; Zhang, W; Samelson, LE

Published Date

  • March 2001

Published In

Volume / Issue

  • 14 / 3

Start / End Page

  • 315 - 329

PubMed ID

  • 11290340

Pubmed Central ID

  • 11290340

International Standard Serial Number (ISSN)

  • 1074-7613

Digital Object Identifier (DOI)

  • 10.1016/s1074-7613(01)00112-1

Language

  • eng

Conference Location

  • United States