Regulation of ZAP-70 intracellular localization: visualization with the green fluorescent protein.

Published

Journal Article

To investigate the cellular dynamics of ZAP-70, we have studied the distribution and regulation of its intracellular location using a ZAP-70 green fluorescent protein chimera. Initial experiments in epithelial cells indicated that ZAP-70 is diffusely located throughout the quiescent cell, and accumulates at the plasma membrane upon cellular activation, a phenotype enhanced by the coexpression of Lck and the initiation of ZAP-70 kinase activity. Subsequent studies in T cells confirmed this phenotype. Intriguingly, a large amount of ZAP-70, both chimeric and endogenous, resides in the nucleus of quiescent and activated cells. Nuclear ZAP-70 becomes tyrosine phosphorylated upon stimulation via the T cell receptor, indicating that it may have an important biologic function.

Full Text

Duke Authors

Cited Authors

  • Sloan-Lancaster, J; Zhang, W; Presley, J; Williams, BL; Abraham, RT; Lippincott-Schwartz, J; Samelson, LE

Published Date

  • November 17, 1997

Published In

Volume / Issue

  • 186 / 10

Start / End Page

  • 1713 - 1724

PubMed ID

  • 9362531

Pubmed Central ID

  • 9362531

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.186.10.1713

Language

  • eng

Conference Location

  • United States