LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway.

Published

Journal Article

In this study, we present the further characterization of a mutant Jurkat T cell line, J.CaM2, that is defective in TCR-mediated signal transduction. Although initial TCR-mediated signaling events such as the inducible tyrosine phosphorylation of the TCR-zeta chain and ZAP-70 are intact in J.CaM2, subsequent events, including increases in intracellular calcium, Ras activation, and IL-2 gene expression are defective. Subsequent analysis of J.CaM2 demonstrated a severe deficiency in pp36/LAT expression, a recently cloned adaptor protein implicated in TCR signaling. Importantly, reexpression of LAT in J.CaM2 restored all aspects of TCR signaling. These results demonstrate a necessary and exclusive role for LAT in T cell activation.

Full Text

Duke Authors

Cited Authors

  • Finco, TS; Kadlecek, T; Zhang, W; Samelson, LE; Weiss, A

Published Date

  • November 1998

Published In

Volume / Issue

  • 9 / 5

Start / End Page

  • 617 - 626

PubMed ID

  • 9846483

Pubmed Central ID

  • 9846483

Electronic International Standard Serial Number (EISSN)

  • 1097-4180

International Standard Serial Number (ISSN)

  • 1074-7613

Digital Object Identifier (DOI)

  • 10.1016/s1074-7613(00)80659-7

Language

  • eng