LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation.

Published

Journal Article

Despite extensive study, several of the major components involved in T cell receptor-mediated signaling remain unidentified. Here we report the cloning of the cDNA for a highly tyrosine-phosphorylated 36-38 kDa protein, previously characterized by its association with Grb2, phospholipase C-gamma1, and the p85 subunit of phosphoinositide 3-kinase. Deduced amino acid sequence identifies a novel integral membrane protein containing multiple potential tyrosine phosphorylation sites. We show that this protein is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules. Its function is demonstrated by inhibition of T cell activation following overexpression of a mutant form lacking critical tyrosine residues. Therefore, we propose to name the molecule LAT-linker for activation of T cells.

Full Text

Duke Authors

Cited Authors

  • Zhang, W; Sloan-Lancaster, J; Kitchen, J; Trible, RP; Samelson, LE

Published Date

  • January 9, 1998

Published In

Volume / Issue

  • 92 / 1

Start / End Page

  • 83 - 92

PubMed ID

  • 9489702

Pubmed Central ID

  • 9489702

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/s0092-8674(00)80901-0

Language

  • eng

Conference Location

  • United States