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Molecular cloning of a novel gene encoding a membrane-associated adaptor protein (LAX) in lymphocyte signaling.

Publication ,  Journal Article
Zhu, M; Janssen, E; Leung, K; Zhang, W
Published in: J Biol Chem
November 29, 2002

Membrane-associated adaptors play an important role in coupling antigen receptor engagement to downstream signaling events, such as Ras-MAPK activation, Ca(2+) flux, and nuclear factor of activated T cells (NFAT) activation. Here we identified a novel membrane-associated adaptor protein, LAX. LAX is mainly expressed in B cells, T cells, and other lymphoid-specific cell types. It shares no overall sequence homology with LAT and is not localized to lipid rafts. However, like LAT, LAX has tyrosine motifs for binding Grb2, Gads, and the p85 subunit of phosphatidylinositol 3-kinase. Upon stimulation via the B or T cell receptors, LAX is rapidly phosphorylated by Src and Syk family tyrosine kinases and interacts with Grb2, Gads, and p85. Overexpression of LAX in Jurkat cells specifically inhibits T cell receptor-mediated p38 MAPK activation and NFAT/AP-1 transcriptional activation. Our data suggested that LAX functions to negatively regulate signaling in lymphocytes.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

November 29, 2002

Volume

277

Issue

48

Start / End Page

46151 / 46158

Location

United States

Related Subject Headings

  • Subcellular Fractions
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Phosphorylation
  • Molecular Sequence Data
  • Mice
  • Membrane Proteins
  • Lymphocytes
  • Humans
  • DNA Primers
 

Citation

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Zhu, M., Janssen, E., Leung, K., & Zhang, W. (2002). Molecular cloning of a novel gene encoding a membrane-associated adaptor protein (LAX) in lymphocyte signaling. J Biol Chem, 277(48), 46151–46158. https://doi.org/10.1074/jbc.M208946200
Zhu, Minghua, Erin Janssen, Kin Leung, and Weiguo Zhang. “Molecular cloning of a novel gene encoding a membrane-associated adaptor protein (LAX) in lymphocyte signaling.J Biol Chem 277, no. 48 (November 29, 2002): 46151–58. https://doi.org/10.1074/jbc.M208946200.
Zhu M, Janssen E, Leung K, Zhang W. Molecular cloning of a novel gene encoding a membrane-associated adaptor protein (LAX) in lymphocyte signaling. J Biol Chem. 2002 Nov 29;277(48):46151–8.
Zhu, Minghua, et al. “Molecular cloning of a novel gene encoding a membrane-associated adaptor protein (LAX) in lymphocyte signaling.J Biol Chem, vol. 277, no. 48, Nov. 2002, pp. 46151–58. Pubmed, doi:10.1074/jbc.M208946200.
Zhu M, Janssen E, Leung K, Zhang W. Molecular cloning of a novel gene encoding a membrane-associated adaptor protein (LAX) in lymphocyte signaling. J Biol Chem. 2002 Nov 29;277(48):46151–46158.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

November 29, 2002

Volume

277

Issue

48

Start / End Page

46151 / 46158

Location

United States

Related Subject Headings

  • Subcellular Fractions
  • Signal Transduction
  • Sequence Homology, Amino Acid
  • Phosphorylation
  • Molecular Sequence Data
  • Mice
  • Membrane Proteins
  • Lymphocytes
  • Humans
  • DNA Primers