An important role of phospholipase Cgamma1 in pre-B-cell development and allelic exclusion.

Published

Journal Article

Phospholipase Cgamma1 (PLCgamma1) has been reported to be expressed predominantly in T cells and to play an important role in T-cell receptor signaling. Here we show that PLCgamma1 is expressed throughout B-cell development, with high expression in B-cell progenitors, and is involved in pre-B-cell receptor (pre-BCR) signaling. Reduced expression of PLCgamma1, in the absence of PLCgamma2 (PLCgamma1+/-PLCgamma2-/-), impedes early B-cell development at the pro-B- to pre-B-cell transition and impairs immunoglobulin heavy chain allelic exclusion, hallmarks of defective pre-BCR signaling. In contrast, early B-cell development is largely normal, whereas late B-cell maturation is impaired in the absence of PLCgamma2 alone (PLCgamma2-/-) and overexpression of PLCgamma1 in PLCgamma2-/- mice fails to restore BCR-mediated B-cell proliferation and maturation. These studies reveal an essential role of PLCgamma1, distinct from that of PLCgamma2, in B-cell development.

Full Text

Duke Authors

Cited Authors

  • Wen, R; Chen, Y; Schuman, J; Fu, G; Yang, S; Zhang, W; Newman, DK; Wang, D

Published Date

  • October 13, 2004

Published In

Volume / Issue

  • 23 / 20

Start / End Page

  • 4007 - 4017

PubMed ID

  • 15372077

Pubmed Central ID

  • 15372077

International Standard Serial Number (ISSN)

  • 0261-4189

Digital Object Identifier (DOI)

  • 10.1038/sj.emboj.7600405

Language

  • eng

Conference Location

  • England