Linker for activation of B cells: a functional equivalent of a mutant linker for activation of T cells deficient in phospholipase C-gamma1 binding.
Adaptor proteins have important functions in coupling stimulation through immunoreceptors with downstream events. The adaptor linker for activation of B cells (LAB)/non-T cell activation linker (NTAL) is expressed in various immune cell types and has a similar domain structure as linker for activation of T cells (LAT). In this study we generated a LAB transgenic mouse to compare the functional differences between LAB and LAT. A LAB transgene expressed in LAT-deficient T cells was able to restore T cell development. However, these mice developed severe organomegaly with disorganized lymphoid tissues. Lymphocytes from these transgenic mice were hyperactivated, and T cells produced large amounts of type II cytokines. In addition, these activities appeared to be uncoupled from the TCR. An examination of the signaling capabilities of these T cells revealed that LAB resembled a LAT molecule unable to bind phospholipase C-gamma1.
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- Type C Phospholipases
- T-Lymphocytes
- Receptors, Antigen, T-Cell
- Phosphoproteins
- Phospholipase C gamma
- Mice, Transgenic
- Mice
- Membrane Proteins
- Lymphocyte Activation
- Jurkat Cells
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Type C Phospholipases
- T-Lymphocytes
- Receptors, Antigen, T-Cell
- Phosphoproteins
- Phospholipase C gamma
- Mice, Transgenic
- Mice
- Membrane Proteins
- Lymphocyte Activation
- Jurkat Cells