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Negative regulation of lymphocyte activation by the adaptor protein LAX.

Publication ,  Journal Article
Zhu, M; Granillo, O; Wen, R; Yang, K; Dai, X; Wang, D; Zhang, W
Published in: J Immunol
May 1, 2005

The membrane-associated adaptor protein LAX is a linker for activation of T cells (LAT)-like molecule that is expressed in lymphoid tissues. Upon stimulation of T or B cells, it is phosphorylated and interacts with Grb2 and the p85 subunit of PI3K. LAX, however, is not capable of replacing LAT in the TCR signaling pathway. In this study we report that upon T or B cell activation, the LAX protein was up-regulated dramatically. Although disruption of the LAX gene by homologous recombination had no major impact on lymphocyte development, it caused a significant reduction in CD23 expression on mature B cells. Interestingly, naive LAX(-/-) mice had spontaneous germinal center formation. Compared with normal T and B cells, LAX(-/-) T and B cells were hyperresponsive and had enhanced calcium flux, protein tyrosine phosphorylation, MAPK and Akt activation, and cell survival upon engagement of the T or B AgRs. Our data demonstrate that LAX functions as a negative regulator in lymphocyte signaling.

Duke Scholars

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

May 1, 2005

Volume

174

Issue

9

Start / End Page

5612 / 5619

Location

United States

Related Subject Headings

  • Up-Regulation
  • T-Lymphocytes
  • Signal Transduction
  • Receptors, Antigen, T-Cell
  • Receptors, Antigen, B-Cell
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Membrane Proteins
  • Lymphocyte Activation
 

Citation

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Zhu, M., Granillo, O., Wen, R., Yang, K., Dai, X., Wang, D., & Zhang, W. (2005). Negative regulation of lymphocyte activation by the adaptor protein LAX. J Immunol, 174(9), 5612–5619. https://doi.org/10.4049/jimmunol.174.9.5612
Zhu, Minghua, Olivia Granillo, Renren Wen, Kaiyong Yang, Xuezhi Dai, Demin Wang, and Weiguo Zhang. “Negative regulation of lymphocyte activation by the adaptor protein LAX.J Immunol 174, no. 9 (May 1, 2005): 5612–19. https://doi.org/10.4049/jimmunol.174.9.5612.
Zhu M, Granillo O, Wen R, Yang K, Dai X, Wang D, et al. Negative regulation of lymphocyte activation by the adaptor protein LAX. J Immunol. 2005 May 1;174(9):5612–9.
Zhu, Minghua, et al. “Negative regulation of lymphocyte activation by the adaptor protein LAX.J Immunol, vol. 174, no. 9, May 2005, pp. 5612–19. Pubmed, doi:10.4049/jimmunol.174.9.5612.
Zhu M, Granillo O, Wen R, Yang K, Dai X, Wang D, Zhang W. Negative regulation of lymphocyte activation by the adaptor protein LAX. J Immunol. 2005 May 1;174(9):5612–5619.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

May 1, 2005

Volume

174

Issue

9

Start / End Page

5612 / 5619

Location

United States

Related Subject Headings

  • Up-Regulation
  • T-Lymphocytes
  • Signal Transduction
  • Receptors, Antigen, T-Cell
  • Receptors, Antigen, B-Cell
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Membrane Proteins
  • Lymphocyte Activation