Targeted gene deletion of heme oxygenase 2 reveals neural role for carbon monoxide.

Journal Article (Journal Article)

Neuronal nitric oxide synthase (nNOS) generates NO in neurons, and heme-oxygenase-2 (HO-2) synthesizes carbon monoxide (CO). We have evaluated the roles of NO and CO in intestinal neurotransmission using mice with targeted deletions of nNOS or HO-2. Immunohistochemical analysis demonstrated colocalization of nNOS and HO-2 in myenteric ganglia. Nonadrenergic noncholinergic relaxation and cyclic guanosine 3',5' monophosphate elevations evoked by electrical field stimulation were diminished markedly in both nNOSDelta/Delta and HO-2(Delta)/Delta mice. In wild-type mice, NOS inhibitors and HO inhibitors partially inhibited nonadrenergic noncholinergic relaxation. In nNOSDelta/Delta animals, NOS inhibitors selectively lost their efficacy, and HO inhibitors were inactive in HO-2(Delta)/Delta animals.

Full Text

Duke Authors

Cited Authors

  • Zakhary, R; Poss, KD; Jaffrey, SR; Ferris, CD; Tonegawa, S; Snyder, SH

Published Date

  • December 23, 1997

Published In

Volume / Issue

  • 94 / 26

Start / End Page

  • 14848 - 14853

PubMed ID

  • 9405702

Pubmed Central ID

  • PMC25126

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.94.26.14848


  • eng

Conference Location

  • United States