c-erbB-2 oncogene expression in hepatocellular carcinoma and cholangiocarcinoma.


Journal Article

The c-erbB-2 proto-oncogene encodes a transmembrane protein which is homologous to the epidermal growth factor receptor. This protein can be localized immunohistochemically in formalin-fixed paraffin-embedded material using a monoclonal antibody NCL-CB11; positive membrane staining correlates with gene amplification and protein overexpression in breast cancer. Using this technique we have shown that only 2/26 (8%) of hepatocellular carcinomas, 0/10 (0%) of cholangiocarcinomas and 0/2 (0%) hepatoblastomas overexpressed c-erbB-2 as evidenced by membrane staining. Moreover c-erbB-2 mRNA was not detected in seven hepatocellular carcinomas examined by Northern blot analysis. c-erbB-2 overexpression is, therefore, unlikely to be contributing to the malignant phenotype in hepatocellular carcinoma and cholangiocarcinoma.

Full Text

Cited Authors

  • Collier, JD; Guo, K; Mathew, J; May, FE; Bennett, MK; Corbett, IP; Bassendine, MF; Burt, AD

Published Date

  • March 1992

Published In

Volume / Issue

  • 14 / 2-3

Start / End Page

  • 377 - 380

PubMed ID

  • 1380026

Pubmed Central ID

  • 1380026

Electronic International Standard Serial Number (EISSN)

  • 1600-0641

International Standard Serial Number (ISSN)

  • 0168-8278

Digital Object Identifier (DOI)

  • 10.1016/0168-8278(92)90186-s


  • eng