Multiple and specific initiation of T4 DNA replication.

Published

Journal Article

Partially replicated T4 DNA molecules (PRM) whose parental or progeny DNA was labeled with bromodeoxyuridine BUdR was analyzed by gradual shearing followed by CsCl banding of the sheared product. Analysis of PRM containing 18-mum replicated DNA showed that each replicated region was 3- to 6-mum long, indicating three to 6 replicative sites per molecule. Analysis of PRM containing 9-mum replicated DNA similarly indicated two to three replicated regions per molecule. DNA from the replicated regions of PRM containing 10-mum replicated DNA ("donor") was hybridized to DNA from mature phage ("recipient"), and the resulting hybrid was subjected to digestion with exonuclease I. The extent of protection of the recipient and more efficient self-annealing of progeny fragments from PRM indicated that the replicated regions represented 8 to 10 nonrandom locations of the genome. Possible significance of multiple sites for initiation of DNA replication is discussed.

Full Text

Duke Authors

Cited Authors

  • Howe, CC; Buckley, PJ; Carlson, KM; Kozinski, AW

Published Date

  • July 1, 1973

Published In

Volume / Issue

  • 12 / 1

Start / End Page

  • 130 - 148

PubMed ID

  • 4579821

Pubmed Central ID

  • 4579821

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States