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A recombinational portrait of the Drosophila pseudoobscura genome.

Publication ,  Journal Article
Ortiz Barrientos, D; Chang, AS; Noor, MAF
Published in: Genet Res
February 2006

Drosophila pseudoobscura has been intensively studied by evolutionary biologists for over 70 years. The recent publication of the genome sequence not only permits studies of comparative genomics with other dipterans but also opens the door to identifying genes associated with adaptive traits or speciation or testing for the signature of natural selection across the genome. Information on regional rates of recombination, localization of inversion breakpoints distinguishing it from its sibling species D. persimilis, and known polymorphic markers may be useful in such studies. Here, we present a molecular linkage map of four of the five major chromosome arms of D. pseudoobscura. In doing so, we order and orient several sequence contig groups, localize the inversion breakpoints on chromosome 2 to intervals of 200 kilobases, and identify one error in the published sequence assembly. Our results show that regional recombination rates in D. pseudoobscura are much higher than in D. melanogaster and significantly higher even than in D. persimilis. Furthermore, we detect a non-significant positive correlation between recombination rate and published DNA sequence variation. Finally, the online Appendix presents 200 primer sequence pairs for molecular markers that can be used for mapping of quantitative trait loci, of which 125 are known to be polymorphic within or between species.

Duke Scholars

Published In

Genet Res

Publication Date

February 2006

Volume

87

Issue

1

Start / End Page

23 / 31

Related Subject Headings

  • Recombination, Genetic
  • Genome
  • Evolutionary Biology
  • Drosophila melanogaster
  • Drosophila
  • Contig Mapping
  • Chromosomes
  • Chromosome Aberrations
  • Animals
  • 3105 Genetics
 

Citation

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ICMJE
MLA
NLM
Ortiz Barrientos, D., Chang, A. S., & Noor, M. A. F. (2006). A recombinational portrait of the Drosophila pseudoobscura genome. Genet Res, 87(1), 23–31.
Ortiz Barrientos, D., A. S. Chang, and M. A. F. Noor. “A recombinational portrait of the Drosophila pseudoobscura genome.Genet Res 87, no. 1 (February 2006): 23–31.
Ortiz Barrientos D, Chang AS, Noor MAF. A recombinational portrait of the Drosophila pseudoobscura genome. Genet Res. 2006 Feb;87(1):23–31.
Ortiz Barrientos, D., et al. “A recombinational portrait of the Drosophila pseudoobscura genome.Genet Res, vol. 87, no. 1, Feb. 2006, pp. 23–31.
Ortiz Barrientos D, Chang AS, Noor MAF. A recombinational portrait of the Drosophila pseudoobscura genome. Genet Res. 2006 Feb;87(1):23–31.

Published In

Genet Res

Publication Date

February 2006

Volume

87

Issue

1

Start / End Page

23 / 31

Related Subject Headings

  • Recombination, Genetic
  • Genome
  • Evolutionary Biology
  • Drosophila melanogaster
  • Drosophila
  • Contig Mapping
  • Chromosomes
  • Chromosome Aberrations
  • Animals
  • 3105 Genetics