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A comprehensive structure-function analysis of Arabidopsis SNI1 defines essential regions and transcriptional repressor activity.

Publication ,  Journal Article
Mosher, RA; Durrant, WE; Wang, D; Song, J; Dong, X
Published in: The Plant cell
July 2006

The expression of systemic acquired resistance (SAR) in plants involves the upregulation of many Pathogenesis-Related (PR) genes, which work in concert to confer resistance to a broad spectrum of pathogens. Because SAR is a costly process, SAR-associated transcription must be tightly regulated. Arabidopsis thaliana SNI1 (for Suppressor of NPR1, Inducible) is a negative regulator of SAR required to dampen the basal expression of PR genes. Whole genome transcriptional profiling showed that in the sni1 mutant, Nonexpresser of PR genes (NPR1)-dependent benzothiadiazole S-methylester-responsive genes were specifically derepressed. Interestingly, SNI1 also repressed transcription when expressed in yeast, suggesting that it functions as an active transcriptional repressor through a highly conserved mechanism. Chromatin immunoprecipitation indicated that histone modification may be involved in SNI1-mediated repression. Sequence comparison with orthologs in other plant species and a saturating NAAIRS-scanning mutagenesis of SNI1 identified regions in SNI1 that are required for its activity. The structural similarity of SNI1 to Armadillo repeat proteins implies that SNI1 may form a scaffold for interaction with proteins that modulate transcription.

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Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

July 2006

Volume

18

Issue

7

Start / End Page

1750 / 1765

Related Subject Headings

  • Transcription, Genetic
  • Structure-Activity Relationship
  • Sequence Analysis, DNA
  • Sequence Alignment
  • Repressor Proteins
  • Protein Conformation
  • Promoter Regions, Genetic
  • Plant Biology & Botany
  • Oligonucleotide Array Sequence Analysis
  • Nuclear Proteins
 

Citation

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Mosher, R. A., Durrant, W. E., Wang, D., Song, J., & Dong, X. (2006). A comprehensive structure-function analysis of Arabidopsis SNI1 defines essential regions and transcriptional repressor activity. The Plant Cell, 18(7), 1750–1765. https://doi.org/10.1105/tpc.105.039677
Mosher, Rebecca A., Wendy E. Durrant, Dong Wang, Junqi Song, and Xinnian Dong. “A comprehensive structure-function analysis of Arabidopsis SNI1 defines essential regions and transcriptional repressor activity.The Plant Cell 18, no. 7 (July 2006): 1750–65. https://doi.org/10.1105/tpc.105.039677.
Mosher RA, Durrant WE, Wang D, Song J, Dong X. A comprehensive structure-function analysis of Arabidopsis SNI1 defines essential regions and transcriptional repressor activity. The Plant cell. 2006 Jul;18(7):1750–65.
Mosher, Rebecca A., et al. “A comprehensive structure-function analysis of Arabidopsis SNI1 defines essential regions and transcriptional repressor activity.The Plant Cell, vol. 18, no. 7, July 2006, pp. 1750–65. Epmc, doi:10.1105/tpc.105.039677.
Mosher RA, Durrant WE, Wang D, Song J, Dong X. A comprehensive structure-function analysis of Arabidopsis SNI1 defines essential regions and transcriptional repressor activity. The Plant cell. 2006 Jul;18(7):1750–1765.

Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

July 2006

Volume

18

Issue

7

Start / End Page

1750 / 1765

Related Subject Headings

  • Transcription, Genetic
  • Structure-Activity Relationship
  • Sequence Analysis, DNA
  • Sequence Alignment
  • Repressor Proteins
  • Protein Conformation
  • Promoter Regions, Genetic
  • Plant Biology & Botany
  • Oligonucleotide Array Sequence Analysis
  • Nuclear Proteins