Review of methods used to study oxygen transport at the microcirculatory level.

Published

Journal Article (Review)

The existence of hypoxic regions in tumors has long been recognized as a key factor leading to radiation resistance. More recently, it has been found that low oxygen levels also affect drug resistance, angiogenesis, cytokine production, cell cycle control, apoptosis, and metastatic propensity of tumors. Until now, most approaches to eliminating hypoxia have been empirical. However, improved understanding of the underlying mechanisms may permit the development of more soundly based, effective approaches. As discussed in this review, critical evaluation of the factors governing oxygen transport in tumors requires a thorough understanding of the methods used to study this process. Many experimental methodologies can be used to address these issues. In this review, the emphasis is placed on techniques that measure parameters on the scale of the diffusion distance of oxygen. Studies at the microregional level provide the most detailed physiological information on such processes. Over the past few years, significant progress in technology has allowed us to measure tumor oxygenation, yet spatial and temporal heterogeneities continue to provide significant challenges to obtaining clear knowledge of oxygen transport.

Full Text

Duke Authors

Cited Authors

  • Dewhirst, MW; Klitzman, B; Braun, RD; Brizel, DM; Haroon, ZA; Secomb, TW

Published Date

  • October 20, 2000

Published In

Volume / Issue

  • 90 / 5

Start / End Page

  • 237 - 255

PubMed ID

  • 11091348

Pubmed Central ID

  • 11091348

International Standard Serial Number (ISSN)

  • 0020-7136

Language

  • eng

Conference Location

  • United States