Circular transcripts of the testis-determining gene Sry in adult mouse testis.

Published

Journal Article

Sry is expressed at higher levels in the adult testis, where no function has been determined, than in the genital ridge, its critical site of action. cDNA and 5' RACE clones isolated from testis or from Sry-transfected cell lines have an unusual structure, with 3' sequences located in a 5' position. RNAase protection assays and reverse transcription polymerase chain reactions confirmed that these unusual RNA molecules represent the most abundant transcript in testis. Furthermore, oligonucleotide hybridization and RNAase H digestion proved that these Sry RNA molecules are circular. Similar transcripts were detected in the testes of mice with Mus musculus musculus, Mus musculus domesticus, and Mus spretus Sry genes. The circular RNA is found in the cytoplasm but is not substantially bound to polysomes. We suggest that the circles arise from normal splicing processes as a consequence of the unusual genomic structure surrounding the Sry locus in the mouse.

Full Text

Duke Authors

Cited Authors

  • Capel, B; Swain, A; Nicolis, S; Hacker, A; Walter, M; Koopman, P; Goodfellow, P; Lovell-Badge, R

Published Date

  • June 1, 1993

Published In

Volume / Issue

  • 73 / 5

Start / End Page

  • 1019 - 1030

PubMed ID

  • 7684656

Pubmed Central ID

  • 7684656

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(93)90279-y

Language

  • eng