Identification of progesterone receptor in human subcutaneous adipose tissue.

Published

Journal Article

Sex steroids are postulated to play a role in adipose tissue regulation and distribution, because the amount and location of adipose tissue changes during puberty and menopause. Because of the nature of adipose tissue, receptors for the female sex steroids have been difficult to demonstrate. To date, estrogen receptor messenger RNA and protein have been identified in human subcutaneous adipose tissue, but the presence of progesterone receptor (PR) has not been reported. In this study, we demonstrate PR message by Northern blot analysis in RNA isolated from the abdominal subcutaneous adipose tissue of premenopausal women. These preliminary studies revealed that PR messenger RNA levels are higher in the stromal-vascular fraction as opposed to the adipocyte fraction. Western blot analysis demonstrates both PR protein isoforms (human PR-A and human PR-B) in human subcutaneous adipose tissue. Using an enzyme-linked immunosorbent assay, total PR could be quantitated. These studies substantiate that sex steroid receptors are present in human adipose tissue, thereby providing a direct route for regulation of adipose tissue by female sex steroids.

Full Text

Duke Authors

Cited Authors

  • O'Brien, SN; Welter, BH; Mantzke, KA; Price, TM

Published Date

  • February 1998

Published In

Volume / Issue

  • 83 / 2

Start / End Page

  • 509 - 513

PubMed ID

  • 9467566

Pubmed Central ID

  • 9467566

International Standard Serial Number (ISSN)

  • 0021-972X

Digital Object Identifier (DOI)

  • 10.1210/jcem.83.2.4561

Language

  • eng

Conference Location

  • United States