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Differential transcriptional activation of peroxisome proliferator-activated receptor gamma by omega-3 and omega-6 fatty acids in MCF-7 cells.

Publication ,  Journal Article
Thoennes, SR; Tate, PL; Price, TM; Kilgore, MW
Published in: Mol Cell Endocrinol
February 25, 2000

While the role of dietary fats in breast cancer remains controversial, the recent cloning of peroxisome proliferator-activated receptor gamma (PPARgamma), a nuclear hormone receptor, from human breast cancer cells lines provides a potential molecular link. Several fatty acids from four classes of dietary fats were tested for their ability to mediate the transcriptional activity of PPARgamma in MCF-7 and MDA-MB-231 cells using growth media with minimal serum. Whereas omega-3 fatty acids inhibit transactivation of PPARgamma to levels below control, omega-6, monounsaturated and saturated fatty acids stimulate the activity of the transcriptional reporter. These studies indicate that individual fatty acids differentially regulate the transcriptional activity of PPARgamma by selectively acting as agonists or antagonists. Furthermore, the transcriptional activation of PPARgamma correlates with cell proliferation in MCF-7 cells. Understanding the effects of individual fats on breast cancer cells and PPARgamma transactivation could provide important new insights into the epidemiology of breast cancer and the role of dietary fat.

Duke Scholars

Published In

Mol Cell Endocrinol

DOI

ISSN

0303-7207

Publication Date

February 25, 2000

Volume

160

Issue

1-2

Start / End Page

67 / 73

Location

Ireland

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcriptional Activation
  • Transcription Factors
  • Receptors, Cytoplasmic and Nuclear
  • Plasmids
  • Peroxisomes
  • Humans
  • Female
  • Fatty Acids, Unsaturated
  • Fatty Acids, Omega-6
 

Citation

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Thoennes, S. R., Tate, P. L., Price, T. M., & Kilgore, M. W. (2000). Differential transcriptional activation of peroxisome proliferator-activated receptor gamma by omega-3 and omega-6 fatty acids in MCF-7 cells. Mol Cell Endocrinol, 160(1–2), 67–73. https://doi.org/10.1016/s0303-7207(99)00254-3
Thoennes, S. R., P. L. Tate, T. M. Price, and M. W. Kilgore. “Differential transcriptional activation of peroxisome proliferator-activated receptor gamma by omega-3 and omega-6 fatty acids in MCF-7 cells.Mol Cell Endocrinol 160, no. 1–2 (February 25, 2000): 67–73. https://doi.org/10.1016/s0303-7207(99)00254-3.
Thoennes SR, Tate PL, Price TM, Kilgore MW. Differential transcriptional activation of peroxisome proliferator-activated receptor gamma by omega-3 and omega-6 fatty acids in MCF-7 cells. Mol Cell Endocrinol. 2000 Feb 25;160(1–2):67–73.
Thoennes, S. R., et al. “Differential transcriptional activation of peroxisome proliferator-activated receptor gamma by omega-3 and omega-6 fatty acids in MCF-7 cells.Mol Cell Endocrinol, vol. 160, no. 1–2, Feb. 2000, pp. 67–73. Pubmed, doi:10.1016/s0303-7207(99)00254-3.
Thoennes SR, Tate PL, Price TM, Kilgore MW. Differential transcriptional activation of peroxisome proliferator-activated receptor gamma by omega-3 and omega-6 fatty acids in MCF-7 cells. Mol Cell Endocrinol. 2000 Feb 25;160(1–2):67–73.
Journal cover image

Published In

Mol Cell Endocrinol

DOI

ISSN

0303-7207

Publication Date

February 25, 2000

Volume

160

Issue

1-2

Start / End Page

67 / 73

Location

Ireland

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcriptional Activation
  • Transcription Factors
  • Receptors, Cytoplasmic and Nuclear
  • Plasmids
  • Peroxisomes
  • Humans
  • Female
  • Fatty Acids, Unsaturated
  • Fatty Acids, Omega-6